Structure of PDB 8q9v Chain A

Receptor sequence

>8q9vA (length=543) Species: 662548 (Variovorax sp. RA8)

TPRNIKAARGTTLRCKGWQQETILRLLENNIENGERPEDLVIYMNAAKAA
RDWDCFDAIVRTLKTMEADETLVVQSGKPVGLFRTHAFAPRVLLANGNVA
GRWAGDANMFELEKRGLTILPGMTAACWQYIGSQGIVQGTYQSFVSAAEQ
YFGGSLAGRIILTAGAGGMGGAQPLAGKMAGAATLVVDVDPVSLERRLNT
GYLDVIATSVDDALARIRTLAAEREGGSVGIVGNAADVFEALHRKELRPD
IVTDQCMVDPYRGYVPSGLSPAEAAQLVRTDPEQALALAAATLARHARAM
LRFRDDGAVVFEYGNTLRARSVAAGVPEAGELPSFVTLFIRPLFCRGIGP
FRWIAASGDPKDIAAIDGIIESTFAEGHMIRQWIPMARKYIQFQGLPARI
GWLGHGERSKLALLVNEAVADGRISAPIAFTRDHLDAGSVASPYRETEKM
QDGSDAVSDWPLLNAMLACSNGASLVALHSNGDKSASAGQTAIADGTPMA
AFKLKSVLDADTGIGVIRYADAGYEVARETRALHGLGIEIGGG

3D structure

• PDB: 8q9v Structure and Substrate Specificity of S -Methyl Thiourocanate Hydratase.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 4.2.1.49: urocanate hydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	O66	A	Y48 M49 M128 D438 R450	Y43 M44 M123 D433 R445
BS02	NAD	A	Y48 M49 N50 I141 G172 G173 M174 D193 V194 S198 N239 A240 Q260 C261 M262 Y269 V270 Y318 N320	Y43 M44 N45 I136 G167 G168 M169 D188 V189 S193 N234 A235 Q255 C256 M257 Y264 V265 Y313 N315

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016153 urocanate hydratase activity
• GO:0016829 lyase activity

Biological Process

• GO:0006547 L-histidine metabolic process
• GO:0006548 L-histidine catabolic process
• GO:0019556 L-histidine catabolic process to glutamate and formamide
• GO:0019557 L-histidine catabolic process to glutamate and formate

External links

• PDB: RCSB:8q9v, PDBe:8q9v, PDBj:8q9v
• PDBsum: 8q9v
• PubMed: 38389448
• UniProt: A0A6P2DXK2