Structure of PDB 8q4d Chain A

Receptor sequence

>8q4dA (length=352) Species: 1422 (Geobacillus stearothermophilus)

MITRGEFFMIKEMYERGMSISDIARELGIDRKTVRKYIHSPNPPSKSKRK
QRKSKLDPFKPYLQKRMLEDGVFNSEKLFFEIRQQGYTGGKTILKDYMKP
FRETAKKKYTVRYETLPGEQMQVDWKEVGEVVIEGKKVKLSLFVATLGYS
RMKYAVFTTSQDQEHLMECLIQSFKYFGGVPKKVLFDNMKTVTDGREQGV
VKWNQRFSEFASYYGFIPKVCRPYRAQTKGKVERAIQYIMDHFYVGTAFE
SIEELNFLLHRWLDQVANRKPNATTGISPQERWAEESLKPLPLKDYDTSY
LSYRKVHWDGSFSYKGEQWLLSAEYAGKEILVKERLNGDIRLYFRGEEIS
HV

3D structure

• PDB: 8q4d Molecular basis for transposase activation by a dedicated AAA+ ATPase.
• Chain: A
• Resolution: 3.62 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	S21 R25 R31 K32 R35 R52 S54 K55 G89 G90 T92 I93 Q161 D162 Q163 K190 D194 N204	S21 R25 R31 K32 R35 R52 S54 K55 G89 G90 T92 I93 Q161 D162 Q163 K190 D194 N204
BS02	dna	A	M1 D30 R49 N74 S75 K99 R102	M1 D30 R49 N74 S75 K99 R102
BS03	dna	A	D187 N188 K190 Y224	D187 N188 K190 Y224
BS04	dna	A	K126 E127 A226 R234 Q237	K126 E127 A226 R234 Q237
BS05	dna	A	T110 V111 R112 Y113 R151 R225 Q227 G230 K231 R234 Y238 K270 N272 A273 T274	T110 V111 R112 Y113 R151 R225 Q227 G230 K231 R234 Y238 K270 N272 A273 T274
BS06	MG	A	D124 D187	D124 D187

Gene Ontology

Molecular Function

• GO:0003677 DNA binding

Biological Process

• GO:0006310 DNA recombination
• GO:0032196 transposition

External links

• PDB: RCSB:8q4d, PDBe:8q4d, PDBj:8q4d
• PDBsum: 8q4d
• PubMed: 38926614
• UniProt: Q45618|TRA6_GEOSE Putative transposase for insertion sequence element IS5376