Structure of PDB 8pzm Chain A

Receptor sequence

>8pzmA (length=499) Species: 652611 (Pseudomonas aeruginosa PA14)

FQGGMEFLVKSVRPETLKTATLVLAVGEGRKLGASAKAVDDATGGAISAV
LKRGDLAGKVGQTLLLQSLPNLKAERVLLVGAGKERELGDRQYRKLASAV
LSTLKGLAGADAALALGDLAVKGRGAHAKARLLVETLADGLYVFDRYKSQ
KAEPLKLKKLTLLADKADSAAVEQGSKEAQAIANGMALTRDLGNLPPNVC
HPTFLGEQAKGLAKEFKSLKVEVLDEKKLRELGMGSFLAVAQGSDQPPRL
IILQYNGAKKDQAPHVLVGKGITFDTGGISLKPGLGMDEMKFDMCGAASV
FGTFRAVLELQLPINLVGLLACAENMPSGGATRPGDIVTTMSGQTVEILN
TDAEGRLVLCDALTYAERFKPQSVIDIATLTGACIVALGSNTSGLMGNNE
ALVRQLLKAGEFADDRAWQLPLFDEYQEQLDSPFADIANIGGPKAGTITA
GCFLSRFAKKYHWAHLDIAGTAWISGGKDKGATGRPVPLLTQYLLERAK

3D structure

• PDB: 8pzm Unveiling the Catalytic Mechanism of a Processive Metalloaminopeptidase.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.11.10: bacterial leucyl aminopeptidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BES	A	K287 D292 M307 D310 D369 E371 T396 L397 T398 W490	K270 D275 M290 D293 D352 E354 T379 L380 T381 W473
BS02	BCT	A	A370 E371 G372 R373 L397	A353 E354 G355 R356 L380
BS03	MN	A	K287 D292 D310 E371	K270 D275 D293 E354
BS04	MN	A	D292 K299 D369 E371	D275 K282 D352 E354

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0008235 metalloexopeptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8pzm, PDBe:8pzm, PDBj:8pzm
• PDBsum: 8pzm
• PubMed: 37924287
• UniProt: Q02RY8|AMPA_PSEAB Probable cytosol aminopeptidase (Gene Name=pepA)