Structure of PDB 8pbq Chain A

Receptor sequence

>8pbqA (length=354) Species: 9606 (Homo sapiens)

KLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPP
IIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYL
NETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRS
VHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGE
VRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLET
MLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWT
IPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDV
QKWP

3D structure

• PDB: 8pbq Beyond genetics: Deciphering the impact of missense variants in CAD deficiency.
• Chain: A
• Resolution: 1.54 Å

Enzymatic activity

• Enzyme Commision number: 2.1.3.2: aspartate carbamoyltransferase.
  3.5.1.2: glutaminase.
  3.5.2.3: dihydroorotase.
  6.3.4.16: carbamoyl-phosphate synthase (ammonia).
  6.3.5.5: carbamoyl-phosphate synthase (glutamine-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NCD	A	H1473 R1475 K1556 T1562 F1563 H1590 V1660 R1661 D1686 A1688 H1690 P1702 G1703	H14 R16 K97 T103 F104 H131 V201 R202 D227 A229 H231 P243 G244
BS02	ZN	A	K1556 H1590 H1614	K97 H131 H155
BS03	ZN	A	H1471 H1473 K1556 D1686	H12 H14 K97 D227
BS04	ZN	A	H1471 C1613 E1637	H12 C154 E178
BS05	ZN	A	E1524 G1804 G1806	E65 G345 G347

Gene Ontology

Molecular Function

• GO:0016787 hydrolase activity
• GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
• GO:0016812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides

External links

• PDB: RCSB:8pbq, PDBe:8pbq, PDBj:8pbq
• PDBsum: 8pbq
• PubMed: 37540500
• UniProt: P27708|PYR1_HUMAN Multifunctional protein CAD (Gene Name=CAD)