Structure of PDB 8p1n Chain A

Receptor sequence

>8p1nA (length=1287)

YIPDYKGMEPIGANIEDLVDYSKDWLSRARNFSFFEVKGTAVFECFNSNE
ANHCQRYPMSRKPRNFLLIQCSLITSYKPATTLSDQIDSRRACSYILNLI
PDTPASYLIHDMAYRYINLTREDMINYYAPRIQFKQTQNVREPGTFKLTS
SMLRAESKAMLDLLNNIESLNIASHIVQSESVSLITKILSDLELNITVLD
KFFQNETQKYLIDVLKKTTAWHIGHLIRDITESLIAHSGLKRSKYWSLHS
YNNGNVILFILPSKSLEVAGSFIRFITVFRIGPGLVDKDNLDTILIDGDS
QWGVSKVMSIDLNRLLALNIAFEKALIATATWFQYYTEDQGQFPLQYAIR
SVFANHFLLAICQKMKLCAIFDNLRYLIPAVTSLYSGFPSLIEKLFERPF
KSSLEVYIYYNIKSLLVALAQNNVYPSFMSRIVYKHYRSLISEVTTCFFL
FEKGLHGNMNEEAKIHLETVEWALKFREKEEKYGESLVENGYMMWELRAN
AELAEQQLYCQDAIELAAIELNKVLATKSSVVANSILSKNWEEPYFSQTR
NISLKGMSGQVQEDGHLSSSVTIIEAIRYLSNSRHNPSLLKLYEETREQK
AMARIVRKYQRTEADRGFFITTLPTRCRLEIIEDYYDAIAKNISEEYISY
GGEKKILAIQGALEKALRWASGESFIELSNHKFIRMKRKLMYVSADATKW
SPGDNSAKFRRFTSMLHNGLPNNKLKNCVIDALKQVYKTDFFMSRKLRNY
IDSMESLDPHIKQFLDFFPDGHHGEVKGNWLQGNLNKCSSLFGVAMSLLF
KQVWTNLFPELDCFFEFAHHSDDALFIYGYLEPVDDGTDWFLFVSQQIQA
GHLHWFSVNTEMWKSMFNLHEHILLLGSIKISPKKTTVSPTNAEFLSTFF
EGCAVSIPFVKILLGSLSDLPGLGYFDDLAAAQSRCVKALDLGASPQVAQ
LAVALCTSKVERLYGTAPGMVNHPAAYLQVKHTDTPIPLGGNGAMSIMEL
ATAGIGMSDKNLLKRALLGYSHKRQKSMLYILGLFKFLMKLSQFSFIGKV
QWKIFTPKSEFEFADMYTSKFLELWSSQHVTYDYIIPKGRDNLLIYLVRK
LNDPSIVTAMTMQSPLQLRFRMQAKQHMKVCRLDGEWVTFREVLAAANSF
AENYSATSQDMDLFQTLTSRAKVARTFTVREKDQIIQNSIPAVIGYKFAV
TVEEMSDVLDTAKFPDSLSVDLKTMKDGVYRELGLDISLPDVMKRIAPML
YKSSKSRVVIVQGNVEGTAEAICRYWLKSMSLVKTIR

3D structure

• PDB: 8p1n Structure of hantaan orthohantavirus (HTNV) polymerase bound to 5'vRNA
• Chain: A
• Resolution: 2.79 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	L388 L389 I402 F524 K558 V559 M560 S561 R566 K616 M617 K653 G733	L163 L164 I167 F272 K306 V307 M308 S309 R314 K364 M365 K401 G457

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004519 endonuclease activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0039689 negative stranded viral RNA replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044220 host cell perinuclear region of cytoplasm

External links

• PDB: RCSB:8p1n, PDBe:8p1n, PDBj:8p1n
• PDBsum: 8p1n
• UniProt: P23456|L_HANTV RNA-directed RNA polymerase L (Gene Name=L)