Structure of PDB 8p1m Chain A

Receptor sequence

>8p1mA (length=1298)

YIPDYKGMEPIGANIEDLVDYSKDWLSRARNFSFFEVKGTAVFECFNSNE
ANHCQRYPMSRKPRNFLLIQCSLITSYKPATTLSDQIDSRRACSYILNLI
PDTPASYLIHDMAYRYINLTREDMINYYAPRIQFKQTQNVREPGTFKLTS
SMLRAESKAMLDLLNIESLNIASHIVQSESVSLITKILSDLELNITDTVL
DKFFQNETQKYLIDVLKKTTAWHIGHLIRDITESLIAHSGLKRSKYWSLH
SYNNGNVILFILPSKSLEVAGSFIRFITVFRIGPGLVDKDNLDTILIDGD
SQWGVSKVMSIDLNRLLALNIAFEKALIATATWFQYYTEDQGQFPLQYAI
RSVFANHFLLAICQKMKLCAIFDNLRYLIPAVTSLYSGFPSLIEKLFERP
FKSSLEVYIYYNIKSLLVALAQNNVYPSFMSRIVYKHYRSLISEVTTCFF
LFEKGLHGNMNEEAKIHLETVEWALKFREKEEKYGESLVENGYMMWELRA
NAELAEQQLYCQDAIELAAIELNKVLATKSSVVANSILSKNWEEPYFSQT
RNISLKGMSGQVQEDGHLSSSVTIIEAIRYLSNSRHNPSLLKLYEETREQ
KAMARIVRKYQRTEADRGFFITTLPTRCRLEIIEDYYDAIAKNISEEYIS
YGGEKKILAIQGALEKALRWASGESFIELSNHKFIRMKRKLMYVSADATK
WSPGDNSAKFRRFTSMLHNGLPNNKLKNCVIDALKQVYKTDFFMSRKLRN
YIDSMESLDPHIKQFLDFFPDGHHGEVKGNWLQGNLNKCSSLFGVAMSLL
FKQVWTNLFPELDCFFEFAHHSDDALFIYGYLEPVDDGTDWFLFVSQQIQ
AGHLHWFSVNTEMWKSMFNLHEHILLLGSIKISPKKTTVSPTNAEFLSTF
FEGCAVSIPFVKILLGSLSDLPGLGYFDDLAAAQSRCVKALDLGASPQVA
QLAVALCTSKVERLYGTAPGMVNHPAAYLQVKHTDTPIPLGGNGAMSIME
LATAGIGMSDKNLLKRALLGYSHKRQKSMLYILGLFKFLMKLSDETFQHE
RLGQFSFIGKVQWKIFTPKSEFEFADMYTSKFLELWSSQHVTYDYIIPKG
RDNLLIYLVRKLNDPSIVTAMTMQSPLQLRFRMQAKQHMKVCRLDGEWVT
FREVLAAANSFAENYSATSQDMDLFQTLTSRAKVARTFTVREKDQIIQNS
IPAVIGYKFAVTVEEMSDVLDTAKFPDSLSVDLKTMKDGVYRELGLDISL
PDVMKRIAPMLYKSSKSRVVIVQGNVEGTAEAICRYWLKSMSLVKTIR

3D structure

• PDB: 8p1m Structure of hantaan orthohantavirus (HTNV) polymerase bound to 5'vRNA and NTP Mg
• Chain: A
• Resolution: 3.23 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	L389 F524 K558 V559 S561 R566 K616 K618 K653 H732 N736	L164 F273 K307 V308 S310 R315 K365 K367 K402 H457 N461
BS02	ATP	A	K884 R892 A973 K975 W976 S977 Q1058 G1059 N1062 D1098	K609 R617 A698 K700 W701 S702 Q783 G784 N787 D823
BS03	MG	A	D972 D1098	D697 D823
BS04	MG	A	D972 D1098 D1099 E1177	D697 D823 D824 E902

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004519 endonuclease activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0039689 negative stranded viral RNA replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044220 host cell perinuclear region of cytoplasm

External links

• PDB: RCSB:8p1m, PDBe:8p1m, PDBj:8p1m
• PDBsum: 8p1m
• UniProt: P23456|L_HANTV RNA-directed RNA polymerase L (Gene Name=L)