Structure of PDB 8oya Chain A

Receptor sequence

>8oyaA (length=455) Species: 192952 (Methanosarcina mazei Go1)

MNPKRIRALKSGKQGDGPVVYWMSRDQRAEDNWALLFSRAIAKEANVPVV
VVFCLTDEFLEAGIRQYEFMLKGLQELEVSLSRKKIPSFFLRGDPGEKIS
RFVKDYNAGTLVTDFSPLRIKNQWIEKVISGISIPFFEVDAHNVVPCWEA
SQKHEYAAHTFRPKLYALLPEFLEEFPELEPNSVTTLSDVLETGVKALLP
ERALLKNKDPLFEPWHFEPGEKAAKKVMESFIADRLDSYGALRNDPTKNM
LSNLSPYLHFGQISSQRVVLEVEKAESNPGSKKAFLDEILIWKEISDNFC
YYNPGYDGFESFPSWAKESLNAHRNDVRSHIYTLEEFEAGKTHDPLWNAS
QMELLSTGKMHGYMRMYWAKKILEWSESPEKALEIAICLNDRYELDGRDP
NGYAGIAWSIGGVHDRAWGEREVTGKIRYMSYEGCKRKFDVKLYIEKYSA
LDKLA

3D structure

• PDB: 8oya Time-resolved crystallography captures light-driven DNA repair
• Chain: A
• Resolution: 2.18 Å

Enzymatic activity

• Enzyme Commision number: 4.1.99.3: deoxyribodipyrimidine photo-lyase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	H161 R256 E301 W305 Y376 M379 W421 W431 R441 Y442 M443 S444 R450 K451	H159 R243 E288 W292 Y363 M366 W408 W418 R428 Y429 M430 S431 R437 K438
BS02	dna	A	Y158 W328 R429 A430 W431 E433 K439 K449 R450 F452	Y156 W315 R416 A417 W418 E420 K426 K436 R437 F439
BS03	FDA	A	Y252 L264 S265 N266 S268 F298 E301 I302 W305 K306 K372 R378 N403 D409 N414 G415 G418	Y239 L251 S252 N253 S255 F285 E288 I289 W292 K293 K359 R365 N390 D396 N401 G402 G405

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003677 DNA binding
• GO:0003904 deoxyribodipyrimidine photo-lyase activity
• GO:0016829 lyase activity

Biological Process

• GO:0000719 photoreactive repair
• GO:0006281 DNA repair

External links

• PDB: RCSB:8oya, PDBe:8oya, PDBj:8oya
• PDBsum: 8oya
• PubMed: 38033070
• UniProt: Q8PYK9