Structure of PDB 8oet Chain A

Receptor sequence

>8oetA (length=457) Species: 192952 (Methanosarcina mazei Go1)

MNPKRIRALKSGKQGDGPVVYWMSRDQRAEDNWALLFSRAIAKEANVPVV
VVFCLTDEFLEAGIRQYEFMLKGLQELEVSLSRKKIPSFFLRGDPGEKIS
RFVKDYNAGTLVTDFSPLRIKNQWIEKVISGISIPFFEVDAHNVVPCWEA
SQKHEYAAHTFRPKLYALLPEFLEEFPELEPNSVTPETLSDVLETGVKAL
LPERALLKNKDPLFEPWHFEPGEKAAKKVMESFIADRLDSYGALRNDPTK
NMLSNLSPYLHFGQISSQRVVLEVEKAESNPGSKKAFLDEILIWKEISDN
FCYYNPGYDGFESFPSWAKESLNAHRNDVRSHIYTLEEFEAGKTHDPLWN
ASQMELLSTGKMHGYMRMYWAKKILEWSESPEKALEIAICLNDRYELDGR
DPNGYAGIAWSIGGVHDRAWGEREVTGKIRYMSYEGCKRKFDVKLYIEKY
SALDKLA

3D structure

• PDB: 8oet Time-resolved crystallography captures light-driven DNA repair
• Chain: A
• Resolution: 2.11 Å

Enzymatic activity

• Enzyme Commision number: 4.1.99.3: deoxyribodipyrimidine photo-lyase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	A160 H161 N257 E301 W305 Y376 M379 W421 R429 W431 R441 Y442 M443 S444 R450 K451	A158 H159 N246 E290 W294 Y365 M368 W410 R418 W420 R430 Y431 M432 S433 R439 K440
BS02	dna	A	Y158 W328 R429 A430 W431 E433 K439 R450 K451 F452	Y156 W317 R418 A419 W420 E422 K428 R439 K440 F441
BS03	FDA	A	Y252 S265 N266 S268 L271 F298 E301 W305 K306 K372 G375 R378 N403 D409 N414 G415 G418	Y241 S254 N255 S257 L260 F287 E290 W294 K295 K361 G364 R367 N392 D398 N403 G404 G407

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003677 DNA binding
• GO:0003904 deoxyribodipyrimidine photo-lyase activity
• GO:0016829 lyase activity

Biological Process

• GO:0000719 photoreactive repair
• GO:0006281 DNA repair

External links

• PDB: RCSB:8oet, PDBe:8oet, PDBj:8oet
• PDBsum: 8oet
• PubMed: 38033070
• UniProt: Q8PYK9