Structure of PDB 8kgq Chain A

Receptor sequence

>8kgqA (length=1190) Species: 10497 (African swine fever virus)

AFEISDFKEHAKKKSMWAGALNKVTISGLMGVFTEDEDLMALPIHRDHCP
ALLKIFDEIIVNATDHERACHNKTKKVTYIKISFDKGVFSCENDGPGIPI
AKHEQASLIAKRDVYVPEVASCHFLAGTNINKAKDCIKGGTNGVGLKLAM
VHSQWAILTTADGAQKYVQHINQRLDIIEPPTITPSREMFTRIELMPVYQ
ELGYAEPLSETEQADLSAWIYLRACQCAAYVGKGTTIYYNDKPCRTGSVM
ALAKMYTLLSAPNSTIHTATIKADAKPYSLHPLQVAAVVSPKFKKFEHVS
VINGVNCVKGEHVTFLKKTINEMVVKKFQQTIKDKNRKTTLRDSCSNIFI
VIVGSIPGIEWTGQRKDELSIAENVFKTHYSIPSSFLTSMTKSIVDILLQ
SISKKDNHKQVDVDKYTRARNAGGKRAQDCMLLAAEGDSALSLLRTGLTL
GKSNPSGPSFDFCGMISLGGVIMNACKKVTNITTDSGETIMVRNEQLTNN
KVLQGIVQVLGLDFNCHYKTQEERAKLRYGCIVACVDQDLDGCGKILGLL
LAYFHLFWPQLIIHGFVKRLLTPLIRVYEKGKTMPVEFYYEQEFDAWAKK
QTSLANHTVKYYKGLAAHDTHEVKSMFKHFDNMVYTFTLDDSAKELFHIY
FGGESELRKRELCTGVVPLTETQTQSIHSVRRIPCSLHLQVDTKAYKLDA
IERQIPNFLDGMTRARRKILAGGVKCFASNNRERKVFQFGGYVADHMFYH
HGDMSLNTSIIKAAQYYPGSSHLYPVFIGIGSFGSRHLGGKDAGSPRYIS
VQLASEFIKTMFPAEDSWLLPYVFEDGQRAEPEYYVPVLPLAIMEYGANP
SEGWKYTTWARQLEDILALVRAYVDKDNPKHELLHYAIKHKITILPLRPS
NYNFKGHLKRFGQYYYSYGTYVISEQRNIITITELPLRVPTVAYIESIKK
SSNRMTFIEEIIDYSSSETIEILVKLKPNSLNRIVEEFKETEEQDSIENF
LRLRNCLHSHLNFVKPKGGIIEFNTYYEILYAWLPYRRELYQKRLMREHA
VLKLRIIMETAIVRYINESAELNLSHYEDEKEASRILSEHGFPPLNHTLI
ISPEFASIEELNQKALQGCYTYILSLQARELLIAAKTRRVEKIKKMQARL
DKVEQLLQESPFPGASVWLEEIDAVEKAIIKGRNTQWKFH

3D structure

• PDB: 8kgq Structure of African swine fever virus topoisomerase II in complex with dsDNA
• Chain: A
• Resolution: 5.6 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	V473 K547 K661 Q706 Y800 P852 S853 E854 G855 W856 K857 S954 R1004 H1010 H1012	V471 K545 K659 Q704 Y798 P850 S851 E852 G853 W854 K855 S952 R1002 H1008 H1010
BS02	dna	A	G472 V473 D543 R705 Q706 T715 A717 R718 H753 A850 N851 P852	G470 V471 D541 R703 Q704 T713 A715 R716 H751 A848 N849 P850

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0000819 sister chromatid segregation
• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8kgq, PDBe:8kgq, PDBj:8kgq
• PDBsum: 8kgq
• PubMed: 38816407
• UniProt: A0A2X0THW2