Structure of PDB 8ke5 Chain A

Receptor sequence
>8ke5A (length=259) Species: 2209 (Methanosarcina mazei) [Search protein sequence]
ALTKSQTDRLEVLLNPKDEISLNSGKPFRELESELLSRRKKDLQQIYAEE
RENYLGKLEREITRFFVDRGFLEIKSPILIPLEYIERMGIDNDTELSKQI
FRVDKNFCLRPMLAPNLYNYLRKLDRALPDPIKIFEIGPCYRKESDGKEH
LEEFTMLGFQQMGSGCTRENLESIITDFLNHLGIDFKIVGDTLDVMHGDL
ELSSAGVGPIPLDREWGIDKPWIGAGFGLERLLKVKHDFKNIKRAARSES
YYNGISTNL
3D structure
PDB8ke5 Rational design of the genetic code expansion toolkit for in vivo encoding of D-amino acids
ChainA
Resolution1.90007 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.26: pyrrolysine--tRNA(Pyl) ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A E396 S399 E201 S204
BS02 ANP A R330 E332 H338 L339 F342 M344 E396 L397 S398 S399 G423 R426 R142 E144 H150 L151 F154 M156 E201 L202 S203 S204 G228 R231
BS03 FXL A L301 A302 M344 F347 Q348 G419 A420 G421 L113 A114 M156 F159 Q160 G224 A225 G226
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0005524 ATP binding
Biological Process
GO:0043039 tRNA aminoacylation

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Molecular Function
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Biological Process
External links
PDB RCSB:8ke5, PDBe:8ke5, PDBj:8ke5
PDBsum8ke5
PubMed37904728
UniProtQ8PWY1|PYLS_METMA Pyrrolysine--tRNA ligase (Gene Name=pylS)

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