Structure of PDB 8k9t Chain A

Receptor sequence

>8k9tA (length=961) Species: 759272 (Thermochaetoides thermophila DSM 1495)

PWRSPWAISVFAFVTSLLGIGLLLAVIHSSVTRQIDPKGCRMSYMRPSYA
KLSDFDTEHTRLASKYSLYLYREQGIDHDVKVRGVPVLFIPGNAGSYKQV
RPIAAEAANYFHDVLQHDEAALRAGVRSLDFFTVDFNEDITAFHGQTLLD
QAEYLNEAIRYILSLYLDPRVSERDPDLPDPTSVIVLGHAMGGIVARTML
IMPNYQHNSINTIITMSAPHARPPVSFDGQIVQTYKDINNYWRHAYSQKW
ANDNPLWHVTLVSIAGGGLDTVVPSDYASIESLVPDTHGFTVFTSTIPNV
WTSMDHQAILWCDQFRKVIIRALFDIVDVHRASQTKPRAQRMRVFKKWFL
SGMETVAEKIAPTSDPTTLLIVDDKSDSITAEGERLVLRELGTQGSVRAH
LMPIPPPGSPELKRFTLLTDTKLDKPGENGKLEVMFCSVIPSQPNPTGPA
IPSQLDLSKGNAGTTRLACTNVAPDVITLPASTRFARFPFSVRKEAEIPP
FSYLEYVLDDISEHQFVAVIEKATIPTPGFVIAEFSDHSNSHHTRHIGLR
NLLTFGISLRLPSNRPMMSEVRIPSVKSSLLAYNLRISALECSGRKDLFA
PLVRQYLAEPYESKYFVNARQAAVSLHGVAPYVPPPMSREPEAEGLAFQL
WTDPTCNSSIQVDLTVDVMGSLGKLYMRYRTVFAAFPLFIVSLVLRKQFQ
VYDSTGSFITFAEGLDLSLRQSIPVMLIVLAALTLSTNFHQNDLLIGTQD
PFFLFLIPLIGIICVGVCTVVNYIALSLTRLISVVISFIGFLTVRFGTAV
LLFLVSTMIPYQLAYLVACLVQLGTLVRAQRISSELRSPANSNFHNYVHS
IFILMLWILPINLPTLVVWMHNLSVHWLTPFTSHHNVFSIMPFILLVETH
TTGQMIPRTCCVLLRHITSILLLSLALYAAVYGVSYAYTLHQFVNLFAFW
LVMVHSTADDW

3D structure

• PDB: 8k9t CryoEM Structure of GLE1-prodS
• Chain: A
• Resolution: 2.66 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	M179 S180 Y181 M182 P184 R238	M42 S43 Y44 M45 P47 R101
BS02	MAN	A	K175 D442 Q444 W448	K38 D305 Q307 W311
BS03	05E	A	S233 R238	S96 R101
BS04	PA1	A	A231 Q444	A94 Q307
BS05	LYI	A	L160 N230 A231 G232 Q236 H443 Y813 Y816 R817 F820 I932	L23 N93 A94 G95 Q99 H306 Y676 Y679 R680 F683 I774
BS06	CLR	A	W143 F150 V928 Y931	W6 F13 V770 Y773
BS07	CLR	A	F280 L1041 P1045 L1048 P1049	F143 L856 P860 L863 P864
BS08	80Y	A	M179 S180 W448	M42 S43 W311

Gene Ontology

Molecular Function

• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0050185 phosphatidylinositol deacylase activity

Biological Process

• GO:0006505 GPI anchor metabolic process
• GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport
• GO:0008218 bioluminescence
• GO:0015031 protein transport

Cellular Component

• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8k9t, PDBe:8k9t, PDBj:8k9t
• PDBsum: 8k9t
• PubMed: 38167496
• UniProt: G0S652