Structure of PDB 8jx8 Chain A

Receptor sequence

>8jx8A (length=1436) Species: 10116 (Rattus norvegicus)

CPTQPFHCPSTQWQCPGYSTCINLSALCDGVFDCPNGTDESPLCNQDSCS
HFNGGCTHQCMQGPFGATCLCPLGYQLANDTKTCEDINECDIPGFCSQHC
VNMRGSFRCACDPEYTLESDGRTCKVTGSENPLLVVASRDKIIVDNITAH
THNLYSLVQDVSFVVALDFDSVTGRVFWSDLLQGKTWSVFQNGTDKRVVH
DSGLSVTEMIAVDWIGRNLYWTDYALETIEVSKIDGSHRTVLISKNVTKP
RGLALDPRMGDNVMFWSDWGHHPRIERASMDGTMRTVIVQEKIYWPCGLS
IDYPNRLIYFMDAYLDYIEFCDYDGHNRRQVIASDLVLHHPHALTLFEDF
VYWTDRGTRQVMQANKWHGGNQSVVMYSVHQPLGITAIHPSRQPPSRNPC
ASASCSHLCLLSAQAPRHYSCACPSGWNLSDDSVNCVRGDQPFLMSVRDN
IIFGISLDPEVKSNDAMVPISGIQHGYDVEFDDSEQFIYWVENPGEIHRV
KTDGSNRTVFAPLSLLGSSLGLALDWVSRNIYYTTPASRSIEVLTLKGDT
RYGKTLIANDGTPLGVGFPVGIAVDPARGKLYWSDHGTDSGVPAKIASAN
MDGTSLKILFTGNLQHLEVVTLDIQEQKLYWAVTSRGVIERGNVDGTERM
ILVHHLAHPWGLVVYGSFLYYSDEQYEVIERVDKSSGNNKVVLRDNVPYL
RGLRVYHRRNAADSSNGCSNNPNACQQICLPVPGGMFSCACASGFKLSPD
GRSCSPYNSFMVVSMLPAVRGFSLELSDHSEAMVPVAGQGRNVLHADVDV
ANGFIYWCDFSSSVRSSNGIRRIKPDGSNFTNVVTYGIGANGIRGVALDW
AAGNLYFTNAFVYETLIEVLRINTTYRRVLLKVSVDMPRHIIVDPKHRYL
FWADYGQKPKIERSFLDCTNRTVLVSEGIVTPRGLAMDHDTGYIYWVDDS
LDLIARIHLDGGESQVVRYGSRYPTPYGITVFGESIIWVDRNLKKVFQAS
KQPGNTDPPVVIRDKINLLRDVTIFDEHAQPLSPAELNNNPCLQSNGGCS
HFCFALPELPTPRCGCAFGTLGNDGKSCATSQEDFLIYSLNNSLRSLHFD
PRDHSLPFQVISVAGTAIALDYDRRNNRIFFTQKLNSLRGQISYVSLYSG
SSSPTVLLSNIGVTDGIAFDWINRRIYYSDFSNQTINSMAEDGSNRAVIA
RVSKPRAIVLDPCRGYMYWTDWGTNAKIERATLGGNFRVPIVNTSLVWPN
GLALDLETDLLYWADASLQKIERSTLTGTNREVVVSTAFHSFGLTVYGQY
IYWTDLYTRKIYRANKYDGSDLVAMTTRLPTQPSGISTVVKTQRQQCSNP
CDQFNGGCSHICAPGPNGAECQCPHEGNWYLANDNKYCVVDTGTRCNQLQ
FTCLNGHCINQDWKCDNDNDCGDGSDELPTVCAFHT

3D structure

• PDB: 8jx8 Cryo-EM structures elucidate the multiligand receptor nature of megalin.
• Chain: A
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	R1556 W1600 H1645 R1661	R251 W295 H340 R356
BS02	peptide	A	F2115 R2149 R2194 R2296 R2325	F810 R844 R889 R991 R1020
BS03	peptide	A	R2511 W2527 W2553 N2555 H2595	R1206 W1222 W1248 N1250 H1290
BS04	CA	A	A1331 D1334 V1336 F1337 D1338 D1344 E1345	A26 D29 V31 F32 D33 D39 E40
BS05	CA	A	D1391 I1392 E1394 N1407 M1408 S1411	D86 I87 E89 N102 M103 S106
BS06	CA	A	D2254 D2257 P2279	D949 D952 P974
BS07	CA	A	W2718 C2720 D2721 D2723 D2725 D2731 E2732	W1413 C1415 D1416 D1418 D1420 D1426 E1427
BS08	CA	A	A1618 D1621 H1644	A313 D316 H339

External links

• PDB: RCSB:8jx8, PDBe:8jx8, PDBj:8jx8
• PDBsum: 8jx8
• PubMed: 38771880
• UniProt: P98158|LRP2_RAT Low-density lipoprotein receptor-related protein 2 (Gene Name=Lrp2)