Structure of PDB 8jtj Chain A

Receptor sequence

>8jtjA (length=1042) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence]

MRYKIGLDIGITSVGWAVMNLDIPRIEDLGVRIFDRAENPQTGESLALPR
RLARSARRRLRRRKHRLERIRRLVIREGILTKEELDKLFEEKHEIDVWQL
RVEALDRKLNNDELARVLLHLAKRRGFKSNRKSKENSTMLKHIEENRAIL
SSYRTVGEMIVKDPKFALHKRNKGENYTNTIARDDLEREIRLIFSKQREF
GNMSCTEEFENEYIAIWASQRPVASKDDIEKKVGFCAFEPKEKRAPKATY
TFQSFIAWEHINKLRLISPSGARGLTDEERRLLYEQAFQKNKITYHDIRT
LLHDTYFKGIVYDRQNENIRFLELDAYHQIRKAVDKVYGKEKSSSFLPID
FDTFGYALTLFKDDADIHSYLRNEYEQNGKRMPNLANKVYDNELIEELLN
LSFTKFGHLSLKALRSILPYMEQGEVYSSACERAGYTFTGPKKKQKTMLL
PNIPPIANPVVMRALTQARKVVNAIIKKYGSPVSIHIELARDLSQTFDER
RKTKKEQDENRKKNETAIRQLMEYGLTLNPTGHDIVKFKLWSEQNGQPIE
IERLLEPGYVEVDAVIPYSRSLDDSYTNKVLVLTREREKGNRIPAEYLGF
ETFVLTNSKKKRDRLLRLHYDENEETEFKNRNLNDTRYISRFFANFIREH
LKFAESDDKQKVYTVNGRVTAHLRSRWEFNKNREESDLHHAVDAVIVACT
TPSDIAKVTAFYQRREQNKELAKKTEPHFPQPWPHFADELRARLSKHPKE
SIKALNLGNYDDQKLESLQPVFVSRMPKRSVTGAAHQETLRRYVGIDERS
GKIQTVVKTKLSEIKLDASGHFPMYGKESDPRTYEAIRQRLLDPKKAFQE
PLYKPKKNGEPGPVIRTVKIIDTKNQVIPLNDGKTVAYNSNIVRVDVFEK
DGKYYCVPVYTMDIMKGILPNKAIEPNKPYSEWKEMTEDYTFRFSLYPND
LIRIELPREKTVKTAAGEEINVKDVFVYYKTIDSANGGLELISHDHRFSL
RGVGSRTLKRFEKYQVDVLGNIYKVRGEKRVGLASSAHSKPG

3D structure

PDB

8jtj Cryo-EM structure of GeoCas9-sgRNA-DNA ternary complex

Chain

Resolution

3.08 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S45 L46 A47 R50 R51 A53 R54 R57 R58 R59 L60 R61 R62 K64 H65 R69 R72 R76 D96 W98 K123 R124 R125 G126 K174 R175 N176 K177 E179 R225 P226 V227 K235 H264 F333 Y439 R475 T478 K489 R661 G813 A814 H816 L820 R822 K838 Y855 E858 K890 K891 P897 V898 I899 R900 T901 K903 D916 T919 M949 Q1049 V1059 R1060 S1069 H1072 S1073 K1074 P1075 G1076	S45 L46 A47 R50 R51 A53 R54 R57 R58 R59 L60 R61 R62 K64 H65 R69 R72 R76 D96 W98 K123 R124 R125 G126 K170 R171 N172 K173 E175 R221 P222 V223 K231 H260 F321 Y427 R463 T466 K477 R631 G783 A784 H786 L790 R792 K808 Y825 E828 K856 K857 P863 V864 I865 R866 T867 K869 D882 T885 M915 Q1015 V1025 R1026 S1035 H1038 S1039 K1040 P1041 G1042
BS02	dna	A	N130 R131 S141 Y181 R248 K267 R269 K315 I317 K374 T416 K417 F418 Y668 Q817 E818 T819 K994 N1020 R1035 G1036 V1037 G1038	N130 R131 S137 Y177 R244 K263 R265 K308 I310 K362 T404 K405 F406 Y638 Q787 E788 T789 K960 N986 R1001 G1002 V1003 G1004
BS03	dna	A	P40 Q41 K908 N909 N925 I926 N961 T1015 D1017 S1018	P40 Q41 K874 N875 N891 I892 N927 T981 D983 S984

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0043571	maintenance of CRISPR repeat elements
GO:0051607	defense response to virus

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:8jtj, PDBe:8jtj, PDBj:8jtj
PDBsum	8jtj
PubMed
UniProt	A0A150MP45

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