Structure of PDB 8jsm Chain A

Receptor sequence

>8jsmA (length=1362) Species: 1570291 (Ebola virus)

QHTQYPDARLSSPIVLDQCDLVTRACGLYSSYSLNPQLRNCKLPKHIYRL
KYDVTVTKFLSDVPVATLPIDFIVPILLKALSGNGFCPVEPRCQQFLDEI
IKYTMQDALFLKYYLKNVGAQEDCVDDHFQEKILSSIQGNEFLHQMFFWY
DLAILTRRGRLNRGNSRSTWFVHDDLIDILGYGDYVFWKIPISLLPLNTQ
GIPHAAMDWYQTSVFKEAVQGHTHIVSVSTADVLIMCKDLITCRFNTTLI
SKIAEVEDPVCSDYPNFKIVSMLYQSGDYLLSILGSDGYKIIKFLEPLCL
AKIQLCSKYTERKGRFLTQMHLAVNHTLEEITEIRALKPSQAHKIREFHR
TLIRLEMTPQQLCELFSIQKHWGHPVLHSETAIQKVKKHATVLKALRPIV
IFETYCVFKYSIAKHYFDSQGSWYSVTSDRNLTPGLNSYIKRNQFPPLPM
IKELLWEFYHLDHPPLFSTKIISDLSIFIKDRATAVERTCWDAVFEPNVL
GYNPPHKFSTKRVPEQFLEQENFSIENVLSYAQKLEYLLPQYRNFSFSLK
EKELNVGRTFGKLPYPTRNVQTLCEALLADGLAKAFPSNMMVVTEREQKE
SLLHQASWHATVRGSSFVTDLEKYNLAFRYEFTAPFIEYCNRCYGVKNVF
NWMHYTIPQCYMHVSDYYNPPHNLTLENRNNPPEGPSSYRGHMGGIEGLQ
QKLWTSISCAQISLVEIKTGFKLRSAVMGDNQCITVLSVFPLETDADEQE
QSAEDNAARVAASLAKVTSACGIFLKPDETFVHSGFIYFGKKQYLNGVQL
PQSLKTATRMAPLSDAIFDDLQGTLASIGTAFERSISETRHIFPCRITAA
FHTFFSVRILQYHHLGFNKGFDLGQLTLGKPLDFGTISLALAVPQVLGGL
SFLNPEKCFYRNLGDPVTSGLFQLKTYLRMIEMDDLFLPLIAKNPGNCTA
IDFVLNPSGLNVPGSQDLTSFLRQIVRRTITLSAKNKLINTLFHASADFE
DEMVCKWLLSSTPVMSRFAADIFSRTPSGKRLQILGYLEGTRTLLASKII
NNNTETPVLDRLRKITLQRWSLWFSYLDHCDNILAEALTQITCTVDLAQI
LREYSWAHILEGRPLIGATLPCMIEQFKVVWLKPYEQCPQCSNAKQPGGK
PFVSVAVKKHIVSAWPNASRISWTIGDGIPGQPAIKPKCPSAALREAIEL
ASRLTWVTQGSSNSDLLIKPFLEARVNLSVQEILQMTPSHYSGNIVHRYN
DQYSPHSFMANRMSNSATRLIVSTNTLGEFSDSNIIFQNVINYAVALFDI
KFRNTEATDIQYNRAHLHLTKCCTREVPAQYLTYTSTLDLDLTRYRENEL
IYDNNPLKGGLN

3D structure

• PDB: 8jsm Molecular mechanism of de novo replication by the Ebola virus polymerase.
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.-
  2.1.1.375: NNS virus cap methyltransferase.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.88: GDP polyribonucleotidyltransferase.
  3.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S14 K48 H49 R166 R170 R485 N501 K510 F511 R515 F563 R571 Y679 G710 R1054 A1058	S11 K45 H46 R163 R167 R482 N498 K507 F508 R512 F560 R568 Y667 G698 R1042 A1046
BS02	ZN	A	C1150 C1153 H1345 H1347	C1138 C1141 H1316 H1318

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
• GO:0005524 ATP binding
• GO:0008168 methyltransferase activity
• GO:0016740 transferase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006370 7-methylguanosine mRNA capping
• GO:0032259 methylation
• GO:0106005 RNA 5'-cap (guanine-N7)-methylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0030430 host cell cytoplasm
• GO:0044423 virion component

External links

• PDB: RCSB:8jsm, PDBe:8jsm, PDBj:8jsm
• PDBsum: 8jsm
• PubMed: 37699521
• UniProt: A0A1C4HDB0