Structure of PDB 8joz Chain A

Receptor sequence

>8jozA (length=257) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQKLRVLD
AGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHC
AAQDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFY
NAHGLLMHNMVAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGW
QIMGKTGVRVFHDYLREKHQQRDCYEALLELETRYCRQEPYITLGRYIHV
TARKPQS

3D structure

PDB

8joz Structural basis for the selective methylation of 5-carboxymethoxyuridine in tRNA modification.

Chain

Resolution

2.22 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K12 F13 N16 I17 K22 R26 A120 W124 Y150 H158 N159 A162 N164 Y167 K176 K177 R178 L180 S181 R209 K218 R246 Y247	K12 F13 N16 I17 K22 R26 A120 W124 Y150 H158 N159 A162 N164 Y167 K176 K177 R178 L180 S181 R209 K218 R246 Y247
BS02	SFG	A	D3 R4 F6 F13 Y18 R26 G52 G53 D73 L74 M78 A101 A102 Q103 H119 A120	D3 R4 F6 F13 Y18 R26 G52 G53 D73 L74 M78 A101 A102 Q103 H119 A120

Gene Ontology

	Molecular Function
GO:0008168	methyltransferase activity
GO:0097697	tRNA (5-carboxymethoxyuridine(34)-5-O)-methyltransferase activity

	Biological Process
GO:0002098	tRNA wobble uridine modification
GO:0006400	tRNA modification
GO:0008033	tRNA processing
GO:0030488	tRNA methylation
GO:0032259	methylation

View graph for
Molecular Function

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Biological Process

External links

PDB	RCSB:8joz, PDBe:8joz, PDBj:8joz
PDBsum	8joz
PubMed	37587716
UniProt	P36566\|CMOM_ECOLI tRNA 5-carboxymethoxyuridine methyltransferase (Gene Name=cmoM)

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