Structure of PDB 8j9x Chain A

Receptor sequence

>8j9xA (length=778) Species: 10497 (African swine fever virus) [Search protein sequence]

VDKYTRARNAGGKRAQDCMLLAAEGDSALSLLRTGLTLGKSNPSGPSFDF
CGMISLGGVIMNACKKVTNITTDSGETIMVRNEQLTNNKVLQGIVQVLGL
DFNCHYKTQEERAKLRYGCIVACVDQDLDGCGKILGLLLAYFHLFWPQLI
IHGFVKRLLTPLIRVYEKGKTMPVEFYYEQEFDAWAKKQTSLVNHTVKYY
KGLAAHDTHEVKSMFKHFDNMVYTFTLDDSAKELFHIYFGGESELRKREL
CTGVVPLTETQTQSIHSVRRIPCSLHLQVDTKAYKLDAIERQIPNFLDGM
TRARRKILAGGVKCFASNNRERKVFQFGGYVADHMFYHHGDMSLNTSIIK
AAQYYPGSSHLYPVFIGIGSFGSRHLGGKDAGSPRYISVQLASEFIKTMF
PAEDSWLLPYVFEDGQRAEPEYYVPVLPLAIMEYGANPSEGWKYTTWARQ
LEDILALVRAYVDKDNPKHELLHYAIKHKITILPLRPSNYNFKGHLKRFG
QYYYSYGTYDISEQRNIITITELPLRVPTVAYIESIKKSSNRMTFIEEII
DYSSSETIEILVKLKPNSLNRIVEEFKETEEQDSIENFLRLRNCLHSHLN
FVKPKGGIIEFNSYYEILYAWLPYRRELYQKRLMREHAVLKLRIIMETAI
VRYINESAELNLSHYEDEKEASRILSEHGFPPLNHTLIISPEFASIEELN
QKALQGCYTYILSLQARELLIAAKTRRVEKIKKMQARLDKVEQLLQESPF
PGASVWLEEIDAVEKAIIKGRNTQWKFH

3D structure

PDB

8j9x Cryo-EM structure of the African swine fever virus topoisomerase 2 complexed with Cut02aDNA and m-AMSA (EDI-3)

Chain

Resolution

3.0 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	V473 D543 R705 Q706 T715 Y751 H753 S761 K764 K793 A850 N851 P852	V59 D129 R291 Q292 T301 Y337 H339 S347 K350 K379 A436 N437 P438
BS02	dna	A	M475 N476 K479 K480 Q498 F653 S657 R660 P852 E854 G855 K857 S954	M61 N62 K65 K66 Q84 F239 S243 R246 P438 E440 G441 K443 S540
BS03	ASW	A	G471 V504	G57 V90

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003916	DNA topoisomerase activity
GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524	ATP binding
GO:0046872	metal ion binding

	Biological Process
GO:0000819	sister chromatid segregation
GO:0006259	DNA metabolic process
GO:0006265	DNA topological change

	Cellular Component
GO:0030430	host cell cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8j9x, PDBe:8j9x, PDBj:8j9x
PDBsum	8j9x
PubMed	38418525
UniProt	Q00942\|TOP2_ASFB7 DNA topoisomerase 2 (Gene Name=TOP)

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