Structure of PDB 8j9v Chain A

Receptor sequence

>8j9vA (length=778) Species: 10497 (African swine fever virus) [Search protein sequence]

VDKYTRARNAGGKRAQDCMLLAAEGDSALSLLRTGLTLGKSNPSGPSFDF
CGMISLGGVIMNACKKVTNITTDSGETIMVRNEQLTNNKVLQGIVQVLGL
DFNCHYKTQEERAKLRYGCIVACVDQDLDGCGKILGLLLAYFHLFWPQLI
IHGFVKRLLTPLIRVYEKGKTMPVEFYYEQEFDAWAKKQTSLVNHTVKYY
KGLAAHDTHEVKSMFKHFDNMVYTFTLDDSAKELFHIYFGGESELRKREL
CTGVVPLTETQTQSIHSVRRIPCSLHLQVDTKAYKLDAIERQIPNFLDGM
TRARRKILAGGVKCFASNNRERKVFQFGGYVADHMFYHHGDMSLNTSIIK
AAQYYPGSSHLYPVFIGIGSFGSRHLGGKDAGSPRYISVQLASEFIKTMF
PAEDSWLLPYVFEDGQRAEPEYYVPVLPLAIMEYGANPSEGWKYTTWARQ
LEDILALVRAYVDKDNPKHELLHYAIKHKITILPLRPSNYNFKGHLKRFG
QYYYSYGTYDISEQRNIITITELPLRVPTVAYIESIKKSSNRMTFIEEII
DYSSSETIEILVKLKPNSLNRIVEEFKETEEQDSIENFLRLRNCLHSHLN
FVKPKGGIIEFNSYYEILYAWLPYRRELYQKRLMREHAVLKLRIIMETAI
VRYINESAELNLSHYEDEKEASRILSEHGFPPLNHTLIISPEFASIEELN
QKALQGCYTYILSLQARELLIAAKTRRVEKIKKMQARLDKVEQLLQESPF
PGASVWLEEIDAVEKAIIKGRNTQWKFH

3D structure

PDB

8j9v Cryo-EM structure of the African swine fever virus topoisomerase 2 complexed with Cut02aDNA and etoposide (EDI-1)

Chain

Resolution

2.71 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	V473 N496 E497 R705 Q706 R718 Y751 H753 S761 K793 A850 N851	V59 N82 E83 R291 Q292 R304 Y337 H339 S347 K379 A436 N437
BS02	dna	A	V473 M475 N476 K479 N502 S657 R660 K661 S797 P852 E854 G855 K857 R1004 H1010	V59 M61 N62 K65 N88 S243 R246 K247 S383 P438 E440 G441 K443 R590 H596
BS03	dna	A	D440 M756	D26 M342
BS04	MG	A	D539 D541	D125 D127
BS05	EVP	A	E438 G439 D440 M756 T760	E24 G25 D26 M342 T346

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003916	DNA topoisomerase activity
GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524	ATP binding
GO:0046872	metal ion binding

	Biological Process
GO:0000819	sister chromatid segregation
GO:0006259	DNA metabolic process
GO:0006265	DNA topological change

	Cellular Component
GO:0030430	host cell cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8j9v, PDBe:8j9v, PDBj:8j9v
PDBsum	8j9v
PubMed	38418525
UniProt	Q00942\|TOP2_ASFB7 DNA topoisomerase 2 (Gene Name=TOP)

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