Structure of PDB 8ip1 Chain A

Receptor sequence

>8ip1A (length=502) Species: 562 (Escherichia coli)

QRFDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSL
WHNVENRQLDAQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYND
AGVDTKQLDLGFAGFRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSA
RGLAIDTYTDSKEEFPDFTAFWFDTVKPGATTFTVYALLDSASITGAYKF
TIHCEKSQVIMDVENHLYARKDIKQLGIAPMTSMFSCGTNERRMCDTIHP
QIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNPKGFGLLQLDRDF
SHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLNNIVCFWQP
EKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFSEGWAPGEH
YPEKWARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDG
YRIQFDWYPTSDSTDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVD
DR

3D structure

• PDB: 8ip1 Novel glycoside hydrolase family enzymes from Escherichia coli associating with osmo-regulated periplasmic glucan synthesis
• Chain: A
• Resolution: 2.06 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	A	Y182 R197 E209 F211 T278 N388 V391	Y136 R151 E163 F165 T232 N342 V345
BS02	BGC	A	G179 Y182 F211	G133 Y136 F165
BS03	BGC	A	F112 L178 Y182 R184 S195 N388	F70 L132 Y136 R138 S149 N342
BS04	BGC	A	M280 D300 E385 T386 L387 N388	M234 D254 E339 T340 L341 N342
BS05	BGC	A	Q191 R359	Q145 R313
BS06	BGC	A	R162 D173 R184 Y192	R116 D127 R138 Y146
BS07	BGC	A	R172 Y190 Q191 Y192	R126 Y144 Q145 Y146

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0030246 carbohydrate binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0016051 carbohydrate biosynthetic process

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:8ip1, PDBe:8ip1, PDBj:8ip1
• PDBsum: 8ip1
• PubMed: 37735577
• UniProt: P40120|OPGD_ECOLI Glucans biosynthesis protein D (Gene Name=mdoD)