Structure of PDB 8ijl Chain A

Receptor sequence

>8ijlA (length=982) Species: 10114 (Rattus)

DDHRLSNTELEQKYGTNIIQGLSSVRATELLARDGPNTLTPPKQTPEIIK
FLKQMVGGFSILLWIGAALCWIAFVIQYVNNSASLDNVYLGAILVLVVIL
TGIFAYYQEAKSTNIMASFSKMIPQQALVIRDAEKKVISAEQLVVGDVVE
IKGGDQIPADIRLVFSQGCKVDNSSLTGESEPQARSTEFTHENPLETKNI
GFYSTTCLEGTATGIVINTGDRTIIGRIASLASGVGSEKTPIAIEIEHFV
HIVAGVAVSIGIIFFITAVCMKYYVLDAIIFLISIIVANVPEGLLATVTV
TLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLW
FDNQIFVADTSENQTKQAFDQSSGTWASLSKIITLCNRAEFRPGQESVPI
MKRTVVGDASETALLKFSEVILGDVMGIRKRNHKVAEIPFNSTNKFQLSI
HETEDPNNKRFLVVMKGAPERILEKCSTIMINGQEQPLDKSSADSFHTAY
MELGGLGERVLGFCHLYLPAEQFPQSYIFDVDSVNFPTSNFCFVGLLSMI
DPPRSTVPDAVSKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANNETVE
DIAKRRNIAVEQVNKREAKAAVVTGMELKDMTPEQLDELLTNYQEIVFAR
TSPQQKLIIVEGCQRQDAIVAVTGDGVNDSPALKKADIGIAMGIAGSDAA
KNAADMVLLDDNFASIVTGVEEGRLIFDNLKKTIAYTLTKNIAELCPFLI
YIVAGLPLPIGTITILFIDLGTDIIPSIALAYEKAESDIMNRKPRHKKKD
RLVNTQLAIYSYLHIGLMQALGGFLVYFTVYAQQGFWPTSLINLRVAWET
DDINDLEDSYGQEWTRYQRKYLEWTGSTAFFVAIMIQQIADLIIRKTRRN
SIFQQGLFRNKVIWVGIASQVIVALILSYGLGSVPALSFTMLRVQYWFVA
VPHAILIWVYDEMRKLFIRLYPGSWWDKNMYY

3D structure

• PDB: 8ijl An unusual conformation from Na + -sensitive non-gastric proton pump mutants reveals molecular mechanisms of cooperative Na + -binding.
• Chain: A
• Resolution: 2.62 Å

Enzymatic activity

• Enzyme Commision number: 7.2.2.13: Na(+)/K(+)-exchanging ATPase.
  7.2.2.19: H(+)/K(+)-exchanging ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ALF	A	G232 D388 T390 T629 K710 N732 D733	G178 D334 T336 T575 K656 N678 D679
BS02	MG	A	D388 T390 D729	D334 T336 D675
BS03	ADP	A	D462 S464 E465 F494 L565	D408 S410 E411 F440 L511
BS04	CLR	A	G309 V310	G255 V256
BS05	Q7G	A	I804 T893 I896	I750 T839 I842
BS06	Q7G	A	R953 F957 Q958 V1013 E1016 R1023 L1024	R899 F903 Q904 V959 E962 R969 L970

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0005215 transporter activity
• GO:0005391 P-type sodium:potassium-exchanging transporter activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008556 P-type potassium transmembrane transporter activity
• GO:0008900 P-type potassium:proton transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006813 potassium ion transport
• GO:0006814 sodium ion transport
• GO:0006883 intracellular sodium ion homeostasis
• GO:0006885 regulation of pH
• GO:0010038 response to metal ion
• GO:0010248 establishment or maintenance of transmembrane electrochemical gradient
• GO:0014070 response to organic cyclic compound
• GO:0030007 intracellular potassium ion homeostasis
• GO:0036376 sodium ion export across plasma membrane
• GO:0055075 potassium ion homeostasis
• GO:1902600 proton transmembrane transport
• GO:1990573 potassium ion import across plasma membrane

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0016323 basolateral plasma membrane
• GO:0016324 apical plasma membrane

External links

• PDB: RCSB:8ijl, PDBe:8ijl, PDBj:8ijl
• PDBsum: 8ijl
• PubMed: 37482134
• UniProt: P54708|AT12A_RAT Potassium-transporting ATPase alpha chain 2 (Gene Name=Atp12a)