Structure of PDB 8idf Chain A

Receptor sequence

>8idfA (length=459) Species: 9606 (Homo sapiens)

DVESLPRGGFRCSLCHVTTANRPSLDAHLGGRKHRHLVELRAARKAQGLR
SVFVSGFPRDVDSAQLSEYFLAFGPVASVVMDKDKGVFAIVEMGDVGARE
AVLSQSQHSLGGHRLRVRPREQKEFQSPDSHQLAKALAEAADVGAQMIKL
VGLRELSEAERQLRSLVVALMQEVFTEFFPGCVVHPFGSSINSFDVHGCD
LALFLDLGAAMLELVGSILRGCVPGVYRVQTVPSARRPVVKFAHRPSGLH
GDVSLSNRLALHNSRFLSLASELDGRVRPLVYTLRAWAQGRGLSGSGPLL
SNYALTLLVIYFLQTRDPPVLPTVSQLTQKAGEGEQVEVDGWDCSFPRDA
SRLEPSINVEPLSSLLAQFFSAVSSWDLRGSLLSLREGQALEGLRLGPLN
LQDPFDLSHNVAANVTSRVAGRLQNCCRAAANYARSLQYQRRSSRGRDWG
LLPLLQPSS

3D structure

• PDB: 8idf Mechanism of U6 snRNA oligouridylation by human TUT1.
• Chain: A
• Resolution: 3.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.19: polynucleotide adenylyltransferase.
  2.7.7.52: RNA uridylyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	V23 N27 S30 A33 H34 G37 R38 H42 R50 Q53 F59 G62 K89 K91 V93 R124 P125 R126 E127 G204 D216 R349 Y356 Q359 V361 K370 R374 D381 N386 A389 N392 P427 Y432 R535 H549 R558	V17 N21 S24 A27 H28 G31 R32 H36 R44 Q47 F53 G56 K83 K85 V87 R118 P119 R120 E121 G188 D200 R220 Y227 Q230 V232 K241 R245 D252 N257 A260 N263 P298 Y303 R395 H409 R418
BS02	ZN	A	C21 H34 H40	C15 H28 H34

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0008270 zinc ion binding

External links

• PDB: RCSB:8idf, PDBe:8idf, PDBj:8idf
• PDBsum: 8idf
• PubMed: 37563152
• UniProt: Q9H6E5|STPAP_HUMAN Speckle targeted PIP5K1A-regulated poly(A) polymerase (Gene Name=TUT1)