Structure of PDB 8i3q Chain A

Receptor sequence

>8i3qA (length=595) Species: 562 (Escherichia coli) [Search protein sequence]

PRPRYREERTLVRKLLPRPGQSKQEFRENVKKLRKAFLQFNADVSGVCQW
AIQFRPRYGKPAEPTETFWKFFLEPETSLPPNDSRSPEFRRLQAFEAAAG
ILDDPAFTNELRDSILAVASRPKTKEAQRLFSRLKDYQPAHRMILAKVAA
EWIESRYRRAHQNWERNYEEWKKEKQEWEQNHPELTPEIREAFNQIFQQL
EVKEKRVRICPAADELYRKWRREYWREPRKPVFRYPSVKRHSIAKIFGEN
YFQADFKNSVVGLRLDSMPAGQYLEFAFAPWPRNYRPQPGETEISSVHLH
FVGTRPRIGFRFRVPHKRSRFDCTQEELDELRSRTFPRKAQDQKFLEAAR
KRLLETFPGNAEQELRLLAVALGTDSARAAFFIGKTFQQAFPLKIVKIEK
LYEQWPNQPRPGLSRDHVGRHLQKMRAQASEIAQKRQELTLQPFDLRGLT
VHTARMIRDWARLNARQIIQLAEENQVDLIVLESLRGFRPPGYENLDQEK
KRRVAFFAHGRIRRKVTEKAVERGMRVVTVPYLASSKVCAECRKKQKDNK
QWEKNKKRGLFKCEGCGSQAQVDENAARVLGRVFWGEIELPTAIP

3D structure

PDB

8i3q Structural transitions upon guide RNA binding and their importance in Cas12g-mediated RNA cleavage.

Chain

Resolution

3.1 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R22 T23 V25 K27 S35 S58 Q62 R68 T142 K143 E144 R147 K221 W367 R368 R376 Y377 S379 K381 P424 Y427 R447 R453 K539 E541 L543 G567 H572 R575 H576 E586 I587 K590 R591 L594 F616 D617 H624 R627 D631 R634 R638	R9 T10 V12 K14 S22 S45 Q49 R55 T124 K125 E126 R129 K203 W225 R226 R234 Y235 S237 K239 P282 Y285 R305 R311 K397 E399 L401 G412 H417 R420 H421 E431 I432 K435 R436 L439 F444 D445 H452 R455 D459 R462 R466
BS02	ZN	A	C711 C735	C539 C563

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0046872	metal ion binding

View graph for
Molecular Function

External links

PDB	RCSB:8i3q, PDBe:8i3q, PDBj:8i3q
PDBsum	8i3q
PubMed	37729124
UniProt	A0A7C2YU39

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