Structure of PDB 8hbh Chain A

Receptor sequence
>8hbhA (length=522) Species: 9606 (Homo sapiens) [Search protein sequence]
SRVYLHTLAESICKLIFPEFERLNVALQRTLAKHKFEKTIAEQAVAAGVP
VEVIKESLGEEVFKICYEEDENILGVVGGTLKDFLNSFSTLLKQASILCL
LHVYYFFPKRTTSLILPGIIKAAAHVLYETEVEVSLYLLYSVHMKPQSSL
VIPTSLFCKTFPFHFMFDKDMTILQFGNGIRRLMNRRDKPNFEEYFEILT
PKINQTFSGIMTMLNMQFVVRVRRWRVMDLKGQMIYIVESSAILFLGSPC
VRGLYLSDIPIHNALRDVVLIGEQARAQDGLKKRLGKLKATLEQAHQALE
EEKKKTVDLLCSIFPCEVAQQLWQGQVVQAKKFSNVTMLFSDIVGFTAIC
SQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHKESDT
HAVQIALMAVKMMELSDEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRY
CLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCPGFVFTPRSREELPP
NFPSEIPGICHFLDANANFLGK
3D structure
PDB8hbh NO binds to the distal site of haem in the fully activated soluble guanylate cyclase.
ChainA
Resolution3.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 4.6.1.2: guanylate cyclase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A D486 D530 D342 D386
BS02 MG A I487 D530 I343 D386
BS03 G2P A G489 F490 T491 I528 R574 G345 F346 T347 I384 R430
Gene Ontology
Molecular Function
GO:0004383 guanylate cyclase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0016829 lyase activity
GO:0016849 phosphorus-oxygen lyase activity
GO:0020037 heme binding
GO:0038023 signaling receptor activity
Biological Process
GO:0006182 cGMP biosynthetic process
GO:0007263 nitric oxide mediated signal transduction
GO:0008015 blood circulation
GO:0008217 regulation of blood pressure
GO:0009190 cyclic nucleotide biosynthetic process
GO:0010750 positive regulation of nitric oxide mediated signal transduction
GO:0019934 cGMP-mediated signaling
GO:0035556 intracellular signal transduction
GO:0038060 nitric oxide-cGMP-mediated signaling
GO:0060087 relaxation of vascular associated smooth muscle
GO:0070482 response to oxygen levels
GO:0098925 retrograde trans-synaptic signaling by nitric oxide, modulating synaptic transmission
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0008074 guanylate cyclase complex, soluble
GO:0098978 glutamatergic synapse
GO:0098982 GABA-ergic synapse

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8hbh, PDBe:8hbh, PDBj:8hbh
PDBsum8hbh
PubMed36972843
UniProtQ02108|GCYA1_HUMAN Guanylate cyclase soluble subunit alpha-1 (Gene Name=GUCY1A1)

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