Structure of PDB 8gy2 Chain A

Receptor sequence

>8gy2A (length=723) Species: 290633 (Gluconobacter oxydans 621H)

QEDTGTAITSSDNGGHPGDWLSYGRSYSEQRYSPLDQINTENVGKLKLAW
HYDLDTNRGQEGTPLIVNGVMYATTNWSKMKALDAATGKLLWSYDPKVPG
NIADRGCCDTVSRGAAYWNGKVYFGTFDGRLIALDAKTGKLVWSVYTIPK
EAQLGHQRSYTVDGAPRIAKGKVLIGNGGAEFGARGFVSAFDAETGKLDW
RFFTVPNPENKPDGAASDDILMSKAYPTWGKNGAWKQQGGGGTVWDSLVY
DPVTDLVYLGVGNGSPWNYKFRSEGKGDNLFLGSIVAINPDTGKYVWHFQ
ETPMDEWDYTSVQQIMTLDMPVNGEMRHVIVHAPKNGFFYIIDAKTGKFI
TGKPYTYENWANGLDPVTGRPNYVPDALWTLTGKPWLGIPGELGGHNFAA
MAYSPKTKLVYIPAQQIPLLYDGQKGGFKAYHDAWNLGLDMNKIGLFDDN
DPEHVAAKKDFLKVLKGWTVAWDPEKMAPAFTINHKGPWNGGLLATAGNV
IFQGLANGEFHAYDATNGNDLYSFPAQSAIIAPPVTYTANGKQYVAVEVG
WGGIYPFLYGGVARTSGWTVNHSRVIAFSLDGKDSLPPKNELGFTPVKPV
PTYDEARQKDGYFMYQTFCSACHGDNAISGGVLPDLRWSGAPRGRESFYK
LVGRGALTAYGMDRFDTSMTPEQIEDIRNFIVKRANESYDDEVKARENST
GVPNDQFLNVPQSTADVPTADHP

3D structure

• PDB: 8gy2 Experimental and Theoretical Insights into Bienzymatic Cascade for Mediatorless Bioelectrochemical Ethanol Oxidation with Alcohol and Aldehyde Dehydrogenases
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 1.1.5.5: alcohol dehydrogenase (quinone).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	A	D138 F652 C653 C656 H657 L667 P668 L670 S673 V686 Y694 M696 F699	D104 F618 C619 C622 H623 L633 P634 L636 S639 V652 Y660 M662 F665
BS02	PQQ	A	C141 C142 D197 G213 A214 E215 W279 N297 D342 K369 F432 W523	C107 C108 D163 G179 A180 E181 W245 N263 D308 K335 F398 W489
BS03	CA	A	E215 N297 D342	E181 N263 D308

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding
• GO:0009055 electron transfer activity
• GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
• GO:0020037 heme binding

Cellular Component

• GO:0016020 membrane
• GO:0030288 outer membrane-bounded periplasmic space

External links

• PDB: RCSB:8gy2, PDBe:8gy2, PDBj:8gy2
• PDBsum: 8gy2
• UniProt: O05542|ADHA_GLUOX Alcohol dehydrogenase (quinone), dehydrogenase subunit (Gene Name=adhA)