Structure of PDB 8gir Chain A

Receptor sequence

>8girA (length=356) Species: 10090 (Mus musculus)

DKLKKVLDKLRLKRKDISEAAETVNKVVERLLRRMQKRESEFKGVEQLNT
GSYYEHVKISAPNEFDVMFKLEVPRIELQEYYETGAFYLVKFKRINPLSH
FLEGEVLSATKMLSKFRKIIKEEVKEIKDIDVSVEKEKPGSPAVTLLIRN
PEEISVDIILALESKGSWPISTKEGLPIQGWLGTKVRTNLRREPFYLVPK
NAKDGNSFQGETWRLSFSHTEKYILNNHGIEKTCCESSGAKCCRKECLKL
MKYLLEQLKKEFQELDAFCSYHVKTAIFHMWTQDPQDSQWDPRNLSSCFD
KLLAFFLECLRTEKLDHYFIPKFNLFSQELIDRKSKEFLSKKIEYERNNG
FPIFDK

3D structure

• PDB: 8gir Structure of Ternary Complex of mouse cGAS with dsDNA and Bound ATP: with 10mM Mg2+ and 0.2mM Mn2+
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.86: cyclic GMP-AMP synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R158 L159 K160 R161 H203 E386 K395	R11 L12 K13 R14 H56 E236 K245
BS02	dna	A	R161 S165 A168 N196 Y200 Y201 K372	R14 S18 A21 N49 Y53 Y54 K222
BS03	dna	A	K335 T338	K185 T188
BS04	dna	A	K315 G316 R342	K165 G166 R192
BS05	ATP	A	G198 S199 E211 D213 R364 S368 E371 K402 S420 Y421	G51 S52 E64 D66 R214 S218 E221 K252 S270 Y271
BS06	ZN	A	H378 C384 C385 C392	H228 C234 C235 C242
BS07	MN	A	E211 D213	E64 D66
BS08	MN	A	E211 D213 D307	E64 D66 D157

External links

• PDB: RCSB:8gir, PDBe:8gir, PDBj:8gir
• PDBsum: 8gir
• PubMed: 38740774
• UniProt: Q8C6L5|CGAS_MOUSE Cyclic GMP-AMP synthase (Gene Name=Cgas)