Structure of PDB 8ghe Chain A

Receptor sequence

>8gheA (length=662) Species: 9606 (Homo sapiens) [Search protein sequence]

GRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDH
DVAEDLGLLQFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQ
GEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPLT
IAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCLE
DFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSRL
VLPSGMEELQAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPLV
MLKMEPNGKLQPMVIQIQPPSPSSPTPTLFLPSDPPLAWLLAKSWVRNSD
FQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINT
RARTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADRG
LLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCRE
ITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWY
AWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQ
PDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEY
LKPSCIENSVTI

3D structure

PDB

8ghe Cryo-EM structures of human arachidonate 12S-lipoxygenase bound to endogenous and exogenous inhibitors.

Chain

Resolution

2.05 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

1.13.11.-
1.13.11.31: arachidonate 12-lipoxygenase.
1.13.11.33: arachidonate 15-lipoxygenase.
3.3.2.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE2	A	H360 H365 H540 N544 I663	H359 H364 H539 N543 I662
BS02	3VV	A	A182 R290 E356 H360 L361 H365 I399 R402 A403 Q406 L407 G411 G412 I413 F414 K416 R585 L589 I593 H596 L597 I663	A181 R289 E355 H359 L360 H364 I398 R401 A402 Q405 L406 G410 G411 I412 F413 K415 R584 L588 I592 H595 L596 I662

Gene Ontology

	Molecular Function
GO:0004052	arachidonate 12(S)-lipoxygenase activity
GO:0005506	iron ion binding
GO:0005515	protein binding
GO:0016165	linoleate 13S-lipoxygenase activity
GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0016787	hydrolase activity
GO:0046872	metal ion binding
GO:0047977	hepoxilin-epoxide hydrolase activity
GO:0050473	arachidonate 15-lipoxygenase activity
GO:0051213	dioxygenase activity

	Biological Process
GO:0001676	long-chain fatty acid metabolic process
GO:0006629	lipid metabolic process
GO:0006631	fatty acid metabolic process
GO:0006690	icosanoid metabolic process
GO:0010656	negative regulation of muscle cell apoptotic process
GO:0019369	arachidonate metabolic process
GO:0019372	lipoxygenase pathway
GO:0019395	fatty acid oxidation
GO:0033559	unsaturated fatty acid metabolic process
GO:0034440	lipid oxidation
GO:0042554	superoxide anion generation
GO:0043651	linoleic acid metabolic process
GO:0051122	hepoxilin biosynthetic process
GO:0061436	establishment of skin barrier
GO:0090331	negative regulation of platelet aggregation
GO:1901751	leukotriene A4 metabolic process
GO:2001303	lipoxin A4 biosynthetic process
GO:2001306	lipoxin B4 biosynthetic process

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0016020	membrane
GO:0042383	sarcolemma
GO:0070062	extracellular exosome

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:8ghe, PDBe:8ghe, PDBj:8ghe
PDBsum	8ghe
PubMed	37506345
UniProt	P18054\|LOX12_HUMAN Polyunsaturated fatty acid lipoxygenase ALOX12 (Gene Name=ALOX12)

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