Structure of PDB 8gcc Chain A

Receptor sequence

>8gccA (length=707) Species: 353153 (Trypanosoma cruzi strain CL Brener)

LGIPKLDDANEAGGKYSHRCTLILTEGDSAKALCTAGLAVKDRDYFGVFP
LRGKPLNVRDATLKKVMACAEFQAVSKIMGLDIRQKYSGVERLRYGHLMI
MSDQDHDGSHIKGLIINMIHHYWPDLIKTPGFLQQFITPIVKARISFFSM
PDYFEWKNAIGDGIRNYEIRYYKGLGTSGAKEGREYFENIDRHRLDFVHE
DATDDARIVMAFAKDKVEERKHWITQFKANTNVNESMNYNVRTVRYSEFV
DKELILFSVADCERSIPSVIDGLKPGQRKIIFSSFKRRLTRSIKVVQLAG
YVSEHAAYHHGEQSLVQTIVGLAQNFVGSNNVPLLQQDGQFGTRLQGGKD
HAAGRYIFTRLTNIARYIYHPSDDFVVDYKDDDGLSVEPFYYVPVIPMVL
VNGTSGIGTGFATNIPNYSPLEVIDNLMRLLRGEEVQPMKPWYFGFAGTI
EEKEKGKFVSTGCANVRPDGVVQITELPIGTWTQGYKKFLEELREKEVVV
QYREHNTDVTVDFEVFLHPEVLHHWVAQGCVEERLQLREYIHATNIIAFD
REGQITKYRDAEAVLKEFYLVRLEYYAKRRDFLIGDLRSVASKLENMVRF
VTEVVDGRLIVTRRRKKELLEELRQRGYAPFPEMRRAARDYDYLLGMRLW
NLTAEMIARLQSQLQKARDELAALEKRTPKDLWAEDLNQLRPRIENLFEE
RAKEIAS

3D structure

• PDB: 8gcc Cyanotriazoles are selective topoisomerase II poisons that rapidly cure trypanosome infections.
• Chain: A
• Resolution: 2.94 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L464 N465 H518 F631 R639 K640 I826 G827 G829 K876 Y959 H961 T963	L56 N57 H110 F212 R220 K221 I407 G408 G410 K457 Y540 H542 T544
BS02	dna	A	D436 R460 R683 Y727 H729 G730 S733 S824 I826	D28 R52 R264 Y308 H310 G311 S314 S405 I407
BS03	YWX	A	R460 C477 E479	R52 C69 E71

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006259 DNA metabolic process
• GO:0006265 DNA topological change

External links

• PDB: RCSB:8gcc, PDBe:8gcc, PDBj:8gcc
• PDBsum: 8gcc
• PubMed: 37384702
• UniProt: Q4DE53