Structure of PDB 8gap Chain A

Receptor sequence

>8gapA (length=1012) Species: 5911 (Tetrahymena thermophila)

KQMLTRKEDLLTVLKQISALKYVSNLYEFLLATEKIVQTSELDTQFQEFL
TTTIIASEQNLVENYKQKYNQPNFSQLTIKQVIDDSIILLGNKQNYVQQI
GTTTIGFYVEYENINLSRQTLYSSNFRNLLNIFGEEDFKYFLIDFLVFTK
VEQNGYLQVAGVCLNQYFSENQYIYPEIQRSQIFYCNHMGREPGVFKSSF
FNYSEPQTIIKKTLLKEYQSKNFSCQEERDLFLEFTEKIVQNFHNINFNY
LLKKFCKLPENYQSLKSQVKQIVQSENKANQQSCENLFNSLYDTEISYKQ
ITNFLRQIIQNCVPNQLLGKKNFKVFLEKLYEFVQMKRFENQKVLDYICF
MDVFDVEWFVDLKNQKFTQKRKYISDKRKILGDLIVFIINKIVIPVLRYN
FYITEKHKEGSQIFYYRKPIWKLVSKLTIVKLEEENLEKVEEKLIPEDSF
QKYPQGKLRIIPKKGSFRPIMTFLRKDKQKNIKLNLNQILMDSQLVFRNL
KDMLGQKIGYSVFDNKQISEKFAQFIEKWKNKGRPQLYYVTLDIKKCYDS
IDQMKLLNFFNQSDLIQDTYFINKYLLFQRNKRPLLQIMDNINFPYYFNL
KERQIAYSLYDDDDQILQKGFKEIQSDDRPFIVINQDKPRCITKDIIHNH
LKHISQYNVISFNKVKFRQKRGIPQGLNISGVLCSFYFGKLEEEYTQFLK
NAEQVNGSINLLMRLTDDYLFISDSQQNALNLIVQLQNCANNNGFMFNDQ
KITTNFQFPQEDYNLEHFKISVQNECQWIGKSIDMNTLEIKSIQKQTQQE
INQTINVAISIKNLKSQLKNKLRSLFLNQLIDYFNPNINSFEGLCRQLYH
HSKATVMKFYPFMTKLFQIDLKKSKQYSVQYGKENTNENFLKDILYYTVE
DVCKILCYLQFEDEINSNIKEIFKNLYSWIMWDIIVSYLKKKKQFKGYLN
KLLQKIRKSRFFYLKEGCKSLQLILSQQKYQLNKKELEAIEFIDLNNLIQ
DIKTLIPKISAK

3D structure

• PDB: 8gap Structure of LARP7 Protein p65-telomerase RNA Complex in Telomerase Revealed by Cryo-EM and NMR.
• Chain: A
• Resolution: 3.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.49: RNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L559 Q563 D816 I877 N904 K919 N926 Q927 K956	L484 Q488 D718 I779 N806 K821 N828 Q829 K858
BS02	rna	A	S179 Y221 H234 M235 R237 F242 K243 S244 N324 Y325 L327 K328 K329 K332 L333 Y337 Q338 L420 D421 C424 D427 V428 F429 Q444 K445 R446 K447 S450 R453 R473 R492 K532 R534 L549 R550 K576 R658 M687 Y694 N697 G774 I909 K910 K913 S914 K917 I967 K971 S972 K973 K990 Y994 Y995 K1040 K1041 Q1052 K1056 F1059 F1060 Y1061	S169 Y175 H188 M189 R191 F196 K197 S198 N249 Y250 L252 K253 K254 K257 L258 Y262 Q263 L345 D346 C349 D352 V353 F354 Q369 K370 R371 K372 S375 R378 R398 R417 K457 R459 L474 R475 K501 R583 M589 Y596 N599 G676 I811 K812 K815 S816 K819 I869 K873 S874 K875 K892 Y896 Y897 K942 K943 Q954 K958 F961 F962 Y963

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003720 telomerase activity
• GO:0003723 RNA binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0042162 telomeric DNA binding
• GO:0046872 metal ion binding
• GO:0070034 telomerase RNA binding

Biological Process

• GO:0007004 telomere maintenance via telomerase

Cellular Component

• GO:0000333 telomerase catalytic core complex
• GO:0000781 chromosome, telomeric region
• GO:0005634 nucleus
• GO:0005694 chromosome
• GO:0005697 telomerase holoenzyme complex
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:8gap, PDBe:8gap, PDBj:8gap
• PDBsum: 8gap
• PubMed: 37330293
• UniProt: O77448|TERT_TETTS Telomerase reverse transcriptase (Gene Name=TERT)