Structure of PDB 8ftm Chain A

Receptor sequence

>8ftmA (length=714) Species: 759272 (Thermochaetoides thermophila DSM 1495)

RLIPPMDVLHQAILEWDIFHEGGYRCGNVSDTYPDPYSYKQTFFPLLINE
AWRSFVTAKDETTSKPFGIKVLSRMTVDKFMEVTAAVPAQISKDRGLTEG
DIVIISKGEDPLNQPQELHCLSRIWKTTYKKDTVEVVYRLNAKGNQILPA
LTPGSEFQVVKITNMTTIEREYAALESLQYYDLMDEILKAQPSPMLTFGD
EAIKAVMDNYQLNPGQARAILNAKENDGFTLIQGPPGTGKTKTIVAMVGC
LLTGVLKLLVCAPSNAAVDELVLRLKAGVKTMNGTFHKIEVLRLGRSDVI
NAAVKDVTLDELVKARMDAELRDQLHKEAGEIKAKLAEIRPQLDAARLSD
DRASAMKLQREFDELKRRQAHIGAKIDAGNTYARETEIKRRQIQQEILDK
AQVLCATLSGSGHEMFKNLNVEFETVIIDEAAQCVELSALIPLKYGCNKC
ILVGDPKQLPPTVLSQSAAKYGYDQSLFVRMQKNHPKDVHLLDMQYRMHP
EISRFPSKEFYEGLLQDGADMARLRLQPWHQSVLLGPYRFFDVKGSQERG
PKNQSLVNEEEVKVAMQLYMRFRSDYRDIDLTGKIGIITPYKAQLQRLRQ
KFVERYGESITEQIEFNTTDAFQGRECEIIIFSCVRASPTGGIGFMTDIR
RMNVGLTRARSSLWILGDSRALVQGEFWAKLIEDAKQRDRYTNGNIMALL
SQPGPRVSLESLAK

3D structure

• PDB: 8ftm Sen1 architecture: RNA-DNA hybrid resolution, autoregulation, and insights into SETX inactivation in AOA2.
• Chain: A
• Resolution: 3.01 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	T1204 N1270 T1273 R1276 P1394 S1395 N1396 G1426 R1427 D1441 S1552 T1605 Q1609 S1698 Y1734 K1735 T1761 D1763 A1764 R1779 G1784 G1785 G1787 F1788	T98 N164 T167 R170 P263 S264 N265 G295 R296 D310 S409 T462 Q466 S555 Y591 K592 T618 D620 A621 R636 G641 G642 G644 F645
BS02	ADP	A	G1343 G1345 K1346 T1347 K1348 Y1639	G237 G239 K240 T241 K242 Y496

Gene Ontology

Molecular Function

• GO:0004386 helicase activity

External links

• PDB: RCSB:8ftm, PDBe:8ftm, PDBj:8ftm
• PDBsum: 8ftm
• PubMed: 37832548
• UniProt: G0S163