Structure of PDB 8fs8 Chain A

Receptor sequence

>8fs8A (length=426) Species: 4932 (Saccharomyces cerevisiae)

GEQWYEKFKPNCLEQVAIHKRKLKDVQEALDAMFLPNAKHRILLLSGPSG
CSKSTVIKELSKILVPKYRQNSHKVTEFRGDCIVNDLPQMESFSEFLKGA
RYLVMSNLSLILIEDLPNVFHIDTRRRFQQLILQWLYSSEPLLPPLVICI
TECEIPENDNNYRKFGIDYTFSAETIMNKEILMHPRLKRIKFNPINSTLL
KKHLKFICVQNMKMLKEKNKWNKRQEVIDYIAQETGDIRSAITTLQFWAT
SSGSLPISTRESTISYFHAIGKVIHGSHSTNNDNEMINNLFENSNNLLSK
EDFKLGILENYNTFNKGEFSISDASSIVDCLSECDNMNGLPESNEYGLRE
VRKTFRNISKQGHNHGTVYFPREWKVRKLQNSFKVQAEDWLNVSLYKYNA
VHSFRNITLEFGYYAPLIRKCQSYKK

3D structure

• PDB: 8fs8 Structures of 9-1-1 DNA checkpoint clamp loading at gaps from start to finish and ramification to biology.
• Chain: A
• Resolution: 3.04 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R83 K84 Y235 K237 F238 E247 K252 N266 Y339 F340 V441 Y442 F443 W447 R450	R21 K22 Y162 K164 F165 E174 K179 N193 Y266 F267 V368 Y369 F370 W374 R377
BS02	dna	A	P161 Q162	P88 Q89
BS03	dna	A	H341 H348 G349 H351 N437 H438	H268 H275 G276 H278 N364 H365
BS04	AGS	A	Y67 F70 P72 A79 G112 S114 K115 S116 T117 E187 T224 H276 R312 I315	Y5 F8 P10 A17 G50 S52 K53 S54 T55 E114 T151 H203 R239 I242
BS05	MG	A	S116 E187	S54 E114

Gene Ontology

Molecular Function

• GO:0003689 DNA clamp loader activity

Biological Process

• GO:0000077 DNA damage checkpoint signaling
• GO:0006281 DNA repair

Cellular Component

• GO:0005634 nucleus
• GO:0031389 Rad17 RFC-like complex

External links

• PDB: RCSB:8fs8, PDBe:8fs8, PDBj:8fs8
• PDBsum: 8fs8
• PubMed: 37205533
• UniProt: P32641|RAD24_YEAST Checkpoint protein RAD24 (Gene Name=RAD24)