Structure of PDB 8fpj Chain A

Receptor sequence
>8fpjA (length=1345) Species: 162145 (human metapneumovirus) [Search protein sequence]
TVNVYLPDSYLKGVISFSETNAIGSCLLKRPYLKNDNTAKVAIENPVIEH
VRLKNAVNSKMKISDYKVVEPVNMQHEIMKNVHSCELTLLKQFLTRSKNI
STLKLNMICDWLQLKSILSFIDVEFIPSWVSNWFSNWYNLNKLILEFRRE
EVIRTGSILCRSLGKLVFIVSSYGCIVKSNKSKRVSFFTYNQLLTWKDVM
LSRFNANFCIWVSNSLNENQEGLGLRSNLQGMLTNKLYETVDYMLSLCCN
EGFSLVKEFEGFIMSEILRITEHAQFSTRFRNTLLNGLTDQLTKLKNKNR
LRVHSTVLENNDYPMYEVVLKLLGDTLRCIKLLINKNLENAAELYYIFRI
FGHPMVDERDAMDAVKLNNEITKILRLESLTELRGAFILRIIKGFVDNNK
RWPKIKNLKVLSKRWTMYFKAKNYPSQLELSEQDFLELAAIQFEQEFSVP
EKTNLEMVLNDKAISPPKRLIWSVYPKNYLPETIKNRYLEETFNASDSLK
TRRVLEYYLKDNKFDQKELKSYVVRQEYLNDKEHIVSLTGKERELSVGRM
FAMQPGKQRQIQILAEKLLADNIVPFFPETLTKYGDLDLQRIMYIARASI
VTDLSKFNQAFRYETTAICADVADELHGTQSLFCWLHLIVPMTTMICAYR
HAPPETKGEYDIDKIEEQSGLYRYHMGGIEGWCQKLWTMEAISLLDVVSV
KTRCQMTSLLNGDNQSIDVSKPVKLSEGLDEVKADYRLAVKMLKEIRDAY
RNIGHKLKEGETYISRDLQFISKVIQSEGVMHPTPIKKVLRVGPWINTIL
DDIKTSAESIGSLCQELEFRGESIIVSLILRNFWLYNLYMHESKQHPLAG
KQLFKQLNKTLTSVQRFFEIKRENEVVDLWMNIPMQFGGGDPVVFYRSFY
RRTPDFLTEAISHVDILLKISANIKNETKVSFFKALLSIEKNERATLTTL
MRDPQAVGSERQAKVTSDINRTAVTSILSLSPNQLFSDSAIHYSRNEEEV
GIIAENITPVYPHGLRVLYESLPFHKAEKVVNMISGTKSITNLLQRTSAI
NGEDIDRAVSMMLENLGLLSRILSVVVDSIEIPIKSNGRLICCQISRTLR
ETSWNNMEIVGVTSPSITTCMDVIYATSSHLKGIIIEKFSTDRTTRGQRG
PKSPWVGSSTQEKKLVPVYNRQILSKQQREQLEAIGKMRWVYKGTPGLRR
LLNKICLGSLGISYKCVKPLLPRFMSVNFLHRLSVSSRPMEFPASVPAYR
TTNYHFDTSPINQALSERFGNEDINLVFQNAISCGISIMSVVEQLTGRSP
KQLVLIPQLEEIDIMPPPVFQGKFNYKLVDKITSDQHIFSPDKID
3D structure
PDB8fpj Conserved allosteric inhibitory site on the respiratory syncytial virus and human metapneumovirus RNA-dependent RNA polymerases.
ChainA
Resolution2.74 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.1.1.375: NNS virus cap methyltransferase.
2.7.7.48: RNA-directed RNA polymerase.
2.7.7.88: GDP polyribonucleotidyltransferase.
3.6.1.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 Y6L A H1263 R1270 I1293 N1294 L1297 D1305 I1306 N1307 L1308 F1310 H1231 R1238 I1261 N1262 L1265 D1273 I1274 N1275 L1276 F1278
Gene Ontology
Molecular Function
GO:0003924 GTPase activity
GO:0003968 RNA-dependent RNA polymerase activity
GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
GO:0005524 ATP binding
GO:0008168 methyltransferase activity
GO:0016787 hydrolase activity
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0001172 RNA-templated transcription
GO:0006370 7-methylguanosine mRNA capping
GO:0032259 methylation
GO:0106005 RNA 5'-cap (guanine-N7)-methylation
Cellular Component
GO:0030430 host cell cytoplasm
GO:0044423 virion component

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8fpj, PDBe:8fpj, PDBj:8fpj
PDBsum8fpj
PubMed37337079
UniProtQ6WB93|L_HMPVC RNA-directed RNA polymerase L (Gene Name=L)

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