Structure of PDB 8fbu Chain A

Receptor sequence

>8fbuA (length=426) Species: 353152 (Cryptosporidium parvum Iowa II)

PHKFWNTQPVVQNDDSSSEYSFGPIEIEPDSFRKEIYKLPDGFSWFDCNL
WDIESQDFEDTYQLLKDHYVEDDDSQFRFNYSKEFLRWALCVPGQKKNWL
VGVRVNETKKMVGFISAIPIKVRIHNCIMNTSVVNFLCVHKKLRSKRLAP
VLIKEITRRIRCEKIFQSIYTCGKNITKPFTIGTYWHRIINVKKLLEAGF
IGIPRNMTMSSLIKYHRIPADKRIEGFRPSVDSDAEQICKLFENYFMKYK
DVSNETMNNLINYDEINHSKELGKQAYMKLDKIEDLQDKITIHQCFNVED
VKHYFTNIDKVIVTYVRENKNKEITDLFSFFIIESTVINNERFPTINIAY
SYFNIANTCSLKELFNEMLITAKNNNCDAFNTLDLMQNLQVIQDSKFIIG
TGRLRYYVFNWKIPQISPSNVGIILF

3D structure

• PDB: 8fbu Crystal structure of Cryptosporidium parvum N-myristoyltransferase with bound myristoyl-CoA
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.3.1.97: glycylpeptide N-tetradecanoyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MYA	A	H42 K43 F44 W45 Y109 V110 V174 N175 L177 C178 V179 R184 S185 R187 A189 P190 I193 T197 I200 F206 S208 Y210 T211 I216 F449	H2 K3 F4 W5 Y69 V70 V134 N135 L137 C138 V139 R144 S145 R147 A149 P150 I153 T157 I160 F166 S168 Y170 T171 I176 F409
BS02	XOL	A	F119 Y225 H227 F240 I386 I388 Y390 N421 T422 L423	F79 Y185 H187 F200 I346 I348 Y350 N381 T382 L383

Gene Ontology

Molecular Function

• GO:0004379 glycylpeptide N-tetradecanoyltransferase activity
• GO:0016746 acyltransferase activity

Biological Process

• GO:0006499 N-terminal protein myristoylation
• GO:0018008 N-terminal peptidyl-glycine N-myristoylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8fbu, PDBe:8fbu, PDBj:8fbu
• PDBsum: 8fbu
• PubMed: 37722671
• UniProt: Q5CV46