Structure of PDB 8epl Chain A

Receptor sequence

>8eplA (length=1274) Species: 9606 (Homo sapiens)

LIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGI
FCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTLRTLRA
VRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLE
FYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECKDWIGPNDGITQ
FDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFF
VLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAE
EVMLAEEIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYA
EFLFLGLFLLEMSLKSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLR
ALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGM
QLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGG
VSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEA
FNQKHALQHHMSVWEQRTSQLRKHMQMIRRACHYIVNLRYFEMCILLVIA
ASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILLW
NILDFVVVVGALVAFALANALRDIKTIKSLRVLRVLRPLKTIKRLPKLKA
VFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEK
ECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQV
LQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQ
EQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVS
PSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVL
KVIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKL
FRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQ
VFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKG
CEPDTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFE
YLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGL
GKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGAD
RQQLDSELQKETLAIWPHLSQKML

3D structure

• PDB: 8epl Structures of the R-type human Ca v 2.3 channel reveal conformational crosstalk of the intracellular segments.
• Chain: A
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	E309 E657 E1372	E218 E485 E795
BS02	CLR	A	F611 I614 P644 M648 M1397 S1400 I1401	F439 I442 P472 M476 M820 S823 I824
BS03	CLR	A	F566 F1299 F1303 I1401	F394 F722 F726 I824
BS04	CLR	A	A1165 R1182 F1189	A601 R618 F625
BS05	CLR	A	N294 V1493 Y1499	N203 V916 Y922
BS06	PT5	A	N381 S538 F540 K581 W586 L589 R590 V593 M597 F1413 F1414	N290 S366 F368 K409 W414 L417 R418 V421 M425 F836 F837

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005245 voltage-gated calcium channel activity
• GO:0005262 calcium channel activity
• GO:0005509 calcium ion binding
• GO:0008331 high voltage-gated calcium channel activity
• GO:0022843 voltage-gated monoatomic cation channel activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0006816 calcium ion transport
• GO:0007268 chemical synaptic transmission
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0098703 calcium ion import across plasma membrane

Cellular Component

• GO:0005886 plasma membrane
• GO:0005891 voltage-gated calcium channel complex
• GO:0016020 membrane
• GO:0034702 monoatomic ion channel complex
• GO:0043025 neuronal cell body
• GO:0045202 synapse

External links

• PDB: RCSB:8epl, PDBe:8epl, PDBj:8epl
• PDBsum: 8epl
• PubMed: 36446785
• UniProt: Q15878|CAC1E_HUMAN Voltage-dependent R-type calcium channel subunit alpha-1E (Gene Name=CACNA1E)