Structure of PDB 8eoh Chain A

Receptor sequence
>8eohA (length=454) Species: 9606 (Homo sapiens) [Search protein sequence]
RQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRMRTKHGDVFTVQLGGQ
YFTFVMDPLSFGSILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDHEMIHS
ASTKHLRGDGLKDLNETMLDSLSFVMLTSKGWSLDASCWHEDSLFRFCYY
ILFTAGYLSLFGYTKDKEQDLLQAGELFMEFRKFDLLFPRFVYSLLWPRE
WLEVGRLQRLFHKMLSVSHKEGISNWLGNMLQFLREQGVPSAMQDKFNFM
MLWASQGNTGPTSFWALLYLLKHPEAIRAVREEATQLGALQHTPVLDSVV
EETLRLRAAPTLLRLVHEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDP
DIHPEPTVFKYDRFLNPNGSRKVDFFKTGKKIHHYTMPWGSGVSICPGRF
FALSEVKLFILLMVTHFDLELVDPDTPLPHVDPQRWGFGTMQPSHDVRFR
YRLH
3D structure
PDB8eoh Pyridine-containing substrate analogs are restricted from accessing the human cytochrome P450 8B1 active site by tryptophan 281.
ChainA
Resolution2.65 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 1.14.14.139: 5beta-cholestane-3alpha,7alpha-diol 12alpha-hydroxylase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEM A H124 A282 S283 N286 P354 P432 W433 G434 S438 C440 P441 A446 H99 A254 S255 N258 P310 P388 W389 G390 S394 C396 P397 A402
BS02 WOL A F49 Y102 Y218 G481 F24 Y77 Y193 G437
Gene Ontology
Molecular Function
GO:0004497 monooxygenase activity
GO:0005506 iron ion binding
GO:0008397 sterol 12-alpha-hydroxylase activity
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0019825 oxygen binding
GO:0020037 heme binding
GO:0033778 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity
GO:0033779 5beta-cholestane-3alpha,7alpha-diol 12alpha-hydroxylase activity
GO:0046872 metal ion binding
Biological Process
GO:0006629 lipid metabolic process
GO:0006694 steroid biosynthetic process
GO:0006699 bile acid biosynthetic process
GO:0016125 sterol metabolic process
GO:0031667 response to nutrient levels
GO:0045797 positive regulation of intestinal cholesterol absorption
GO:0070723 response to cholesterol
Cellular Component
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8eoh, PDBe:8eoh, PDBj:8eoh
PDBsum8eoh
PubMed36806682
UniProtQ9UNU6|CP8B1_HUMAN 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (Gene Name=CYP8B1)

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