Structure of PDB 8ekg Chain A

Receptor sequence
>8ekgA (length=558) Species: 1547922 (Piscinibacter sakaiensis) [Search protein sequence]
ASRAACEALKDGNGDMVWPNAATVVEVAAWRDAAPATASAAALPEHCEVS
GAIAKRTGIDGYPYEIKFRLRMPAEWNGRFFMEGGSGTNGSLSAATGSIG
GGQIASALSRNFAVIATDGGHDNAVNDNPDALGTVAFGLDPQARLDYGYN
SYDQVTQAGKAAVARFYGRAADKSYFIGCSEGGREGMMLSQRFPSHYDGI
VAGAPGFQLPKAGISGAWTTQSLAPAAVGLDAQGVPLINKSFSDADLHLL
SQAILGTCDALDGLADGIVDNYRACQAAFDPATAANPANGQALQCVGAKT
ADCLSPVQVTAIKRAMAGPVNSAGTPLYNRWAWDAGMSGLSGTTYNQGWR
SWWLGSFNSSANNAQRVSGFSARSWLVDFATPPEPMPMTQVAARMMKFDF
DIDPLKIWATSGQFTQSSMDWHGATSTDLAAFRDRGGKMILYHGMSDAAF
SALDTADWYERLGAAMPGAAGFARLFLVPGMNHCSGGPGTDRFDMLTPLV
AWVERGEAPDQISAWSGTPGYFGVAARTRPLCPYPQIARYKGSGDINTEA
NFACAAPP
3D structure
PDB8ekg MHETase protein engineering using a novel colorimetric assay
ChainA
Resolution2.65 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.1.102: mono(ethylene terephthalate) hydrolase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 CA A D304 D307 L309 D311 I313 D259 D262 L264 D266 I268
BS02 EDO A P250 G251 F252 Q253 Y373 F495 D499 P205 G206 F207 Q208 Y328 F450 D454
Gene Ontology
Molecular Function
GO:0046872 metal ion binding
GO:0052689 carboxylic ester hydrolase activity
Biological Process
GO:0042178 xenobiotic catabolic process
Cellular Component
GO:0009279 cell outer membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8ekg, PDBe:8ekg, PDBj:8ekg
PDBsum8ekg
PubMed38885634
UniProtA0A0K8P8E7|MHETH_PISS1 Mono(2-hydroxyethyl) terephthalate hydrolase (Gene Name=ISF6_0224)

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