Structure of PDB 8efk Chain A

Receptor sequence

>8efkA (length=598) Species: 8187 (Lates calcarifer)

TFSIIDVASDRRLFNTFIKEWKTKERYSLALACEKREHDSDGLVLIGLSV
CWGARDSYYISLQQVSERLGQVRSCLSRPGGVVVTYDIIQVYKTLVLSCG
ISLAGNCEDPKVACWLLDPGSEERTLPNMVTVYCPEELPLLDGLGSAHAH
CPRVRAATKSVLVHAVMNHLTGLLEKDSMLDLFRSIEMPSQVCLALLELN
GVGFSVEECERQKHVMQAKLTALESQAYNLAGHSFSLTSIDDIAQVLFLE
LHLPFSTTKDILEKLRPLHPLPGVILEWRRITNALTKVVFPLQREKQYHP
TLAMDRIYPIAQTHTATGRVSFTEPNIQNVPKDFEICMAFSVSMRHAFVP
FSGGMILAADYSQLELRVLAHLSKDQRLLQVLNGGADVFRCIAAEWKGVD
PETVNDSLRQQAKQICYGIIYGMGAKSLGEQMGVEENDAACYIESFKARY
KGINAFLKETVKNCIKNGYVQTLMGRRRYLPGISNTNTHIKAHAERQAVN
TTVQGSAADIVKLATVNIQKRLRKTYPTAPLSHQHTLRGAFFVLQLHDEL
IYETREEDLIQVAQIVKREMESAVKLYVKLKAKVKVGPSWGNLQDLDL

3D structure

• PDB: 8efk Structural basis of DNA polymerase theta mediated DNA end joining.
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T2321 K2322 T2380 R2382 Q2391 N2392 P2394 K2395 R2448 G2521 Y2524 M2526 G2527 S2530 R2581 H2592 H2596 R2599 Q2600 N2603 Q2607 L2662	T258 K259 T317 R319 Q328 N329 P331 K332 R345 G418 Y421 M423 G424 S427 R478 H489 H493 R496 Q497 N500 Q504 L546
BS02	MG	A	D2463 Y2464 D2664	D360 Y361 D548
BS03	DDS	A	Q2466 E2468 R2512 K2516 Q2517 Y2520 Y2524	Q363 E365 R409 K413 Q414 Y417 Y421

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:8efk, PDBe:8efk, PDBj:8efk
• PDBsum: 8efk
• PubMed: 36583344
• UniProt: A0A4W6BKE5