Structure of PDB 8efc Chain A

Receptor sequence

>8efcA (length=595) Species: 8187 (Lates calcarifer)

TFSIIDVASDRRLFNTFIKEWKTKERYSLALACEKREHDSDGLVLIGLSV
CWGARDSYYISLQQVSERLGQVRSCLSRPGGVVVTYDIIQVYKTLVLSCG
ISLAGNCEDPKVACWLLDPGSEERTLPNMVTVYCPEELPLLDGLAHCPRV
RAATKSVLVHAVMNHLTGLLEKDSMLDLFRSIEMPSQVCLALLELNGVGF
SVEECERQKHVMQAKLTALESQAYNLAGHSFSLTSIDDIAQVLFLELHLP
QFSTTKDILEKLRPLHPLPGVILEWRRITNALTKVVFPLQREKQYHPTLA
MDRIYPIAQTHTATGRVSFTEPNIQNVPKDFEICMAFSVSMRHAFVPFSG
GMILAADYSQLELRVLAHLSKDQRLLQVLNGGADVFRCIAAEWKGVDPET
VNDSLRQQAKQICYGIIYGMGAKSLGEQMGVEENDAACYIESFKARYKGI
NAFLKETVKNCIKNGYVQTLMGRRRYLPGISNTNTHIKAHAERQAVNTTV
QGSAADIVKLATVNIQKRLRKTYPTAPLSHQHTLRGAFFVLQLHDELIYE
TREEDLIQVAQIVKREMESAVKLYVKLKAKVKVGPSWGNLQDLDL

3D structure

• PDB: 8efc Structural basis of DNA polymerase theta mediated DNA end joining.
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	T2378 A2379 T2380 S2384 T2386 G2521 Y2524 M2526 G2527 K2529 S2530 R2581 A2595 H2596 R2599 Q2600 N2603 Q2607	T312 A313 T314 S318 T320 G415 Y418 M420 G421 K423 S424 R475 A489 H490 R493 Q494 N497 Q501
BS02	dna	A	T2321 K2322 R2343 P2394 K2395 R2448	T255 K256 R277 P328 K329 R342
BS03	DGT	A	R2382 Y2464 Q2466 E2468 R2512 K2516 Q2517 Y2520 Y2524 D2664	R316 Y358 Q360 E362 R406 K410 Q411 Y414 Y418 D545
BS04	MG	A	D2463 Y2464 D2664	D357 Y358 D545

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:8efc, PDBe:8efc, PDBj:8efc
• PDBsum: 8efc
• PubMed: 36583344
• UniProt: A0A4W6BKE5