Structure of PDB 8eey Chain A

Receptor sequence

>8eeyA (length=1544) Species: 45657 (Desulfonema ishimotonii)

TTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNTK
GRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQL
RQRSTLRWTDKNPCPDNAETYCPFCELLGRSWRFRIHFGNLSLPGKPDFD
GPKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDNKVSA
EARKLLCDSLKFTDRLCGALCVIRFNLAEKTAEQIISILDDNKKTEYTRL
LADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGKSVTIRQILTTSADTK
ELKNAGKWREFCEKLGEALYLKSKDMSGGLKITRRILGDAEFHGKPDRLE
KSRSVSIGSVLKETVVCGELVAKTPFFFGAIDEDAKQTALQVLLTPDNKY
RLPRSAVRGILRRDLQTYFDSPCNAELGGRPCMCKTCRIMRGITVMDARS
EYNAPPEIRHRTRINPFTGTVAEGALFNMEVAPEGIVFPFQLRYRGSEDG
LPDALKTVLKWWAEGQAFMSGAASTGKGRFRMENAKYETLDLSDENQRND
YLKNWGWRDEKGLEELKKRLNSGLPEPGNYRDPKWHEINVSIEMASPFIN
GDPIRAAVDKRGTAVVTFVKYKAEGEEAKPVCAYKAESFRGVIRSAVARI
HMEDGVPLTELTHSDCECLLCQIFGSEYEAGKIRFEDLVFESDPEPVTFD
HVAIDRFTGGAADKKKFDDSPLPGSPARPLMLKGSFWIRRDVLEDEEYCK
ALGKALADVNNGLYPLGGKSAIGYGQVKSLGIKGDDKRISRLMNVAVPEK
PKTDAEVRIEAEKVYYPHYFVEPHKKVEREEKPCGHQKFHEGRLTGKIRC
KLITKTPLIVPDTSNDDFFRPEYHKSYAFFRLHKQIMIPGSELRGMVSSV
YETVTNSCFRIFDETKRLSWRMDADHQNVLQDFLPGRVTADGKHIQKFSE
TARVPFYDKTQKHFDILDEQEIAGEKPVRMWVKRFIKRLSLVDPAKHPQK
KQDNKWKRRKEGIATFIEQKNGSYYFNVVTNNGCTSFHLWHKPDNFDQEK
LEGIQNGEKLDCWVRDSRYQKAFQEIPENDPDGWECKEGYLHVVGPSKVE
FSDKKGDVINNFQGTLPSVPNDWKTIRTNDFKNRKRKNEPVFCCEDDKGN
YYTMAKYCETFFFDLKENEEYEIPEKARIKYKELLRVYNNNPQAVPESVF
QSRVARENVEKLKSGDLVYFKHNEKYVEDIVPVRISRTVDDRMIGKRMSA
DLRPCHGDWVEDGDLSALNAYPEKRLLLRHPKGLCPACRLFGTGSYKGRV
RFGFASLENDPEWLIPGKNPGDPFHGGPVMLSLLERPRPTWSIPGSDNKF
KVPGRKFYVHHHAWKTIKDGNHPTTGKAIEQSPNNRTVEALAGGNSFSFE
IAFENLKEWELGLLIHSLQLEKGLAHKLGMAKSMGFGSVEIDVESVRLRK
DWKQWRNGNSEIPNWLGKGFAKLKEWFRDELDFIENLKKLLWFPEGDQAP
RVCYPMLRKKDDPNGNSGYEELKDGEFKKEDRQKKLTTPWTPWA

3D structure

• PDB: 8eey RNA-activated protein cleavage with a CRISPR-associated endopeptidase.
• Chain: A
• Resolution: 2.53 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E13 R16 R35 H43 R53 Y55 T59 L60 R62 S63 R67 K89 F90 D91 T92 R97 L98 Q100 R102 Q103 R104 L129 F146 H149 F150 N152 V172 N174 R175 V176 G181 A183 D185 F186 F187 R227 L232 F382 G384 G419 R444 S445 R448 G449 R452 R453 L467 G468 G469 P471 T484 V485 R501 T502 R503 I504 L516 F517 G561 A562 S564 G641 P643 E677 S678 R680 G681 S685 S716 A720 H741 V742 A743 I744 R746 G749 K756 F757 G808 P908 T910 S948 E949 R951 G952 R967 R978 R1010 S1124 R1125 S1154 K1155 N1195 E1196 K1213 Y1214 C1215 Y1245 N1248 Q1250 S1259 R1291 I1292 R1294 G1349 T1350 G1351 G1355 L1391 E1392 R1393 P1394 R1395 T1397 W1398 Y1415 G1486 M1487 A1488 K1489 Y1561 L1564 Y1576 E1577	E12 R15 R34 H42 R52 Y54 T58 L59 R61 S62 R66 K88 F89 D90 T91 R96 L97 Q99 R101 Q102 R103 L128 F134 H137 F138 N140 V160 N162 R163 V164 G169 A171 D173 F174 F175 R215 L220 F342 G344 G379 R404 S405 R408 G409 R412 R413 L427 G428 G429 P431 T444 V445 R461 T462 R463 I464 L476 F477 G521 A522 S524 G601 P603 E637 S638 R640 G641 S645 S676 A680 H701 V702 A703 I704 R706 G709 K716 F717 G768 P861 T863 S891 E892 R894 G895 R910 R921 R953 S1067 R1068 S1097 K1098 N1138 E1139 K1156 Y1157 C1158 Y1188 N1191 Q1193 S1202 R1234 I1235 R1237 G1292 T1293 G1294 G1298 L1334 E1335 R1336 P1337 R1338 T1340 W1341 Y1358 G1429 M1430 A1431 K1432 Y1504 L1507 Y1519 E1520
BS02	rna	A	K182 Y281 R283 H306 Y313 K364 T373 R375 V511 G514 L516 F517 D705 E717 Y718 D753 K754 K756 F757 D980 H983 Q984 R1125 Q1127 K1155 E1157 S1159 Q1250 L1391 E1392 R1393 N1441 R1443 R1565	K170 Y247 R249 H272 Y279 K324 T333 R335 V471 G474 L476 F477 D665 E677 Y678 D713 K714 K716 F717 D923 H926 Q927 R1068 Q1070 K1098 E1100 S1102 Q1193 L1334 E1335 R1336 N1384 R1386 R1508
BS03	ZN	A	C86 C123 C126	C85 C122 C125
BS04	ZN	A	C472 C474 C477	C432 C434 C437
BS05	ZN	A	H703 C706 C708 C711	H663 C666 C668 C671
BS06	ZN	A	C965 C1312 C1342 C1345	C908 C1255 C1285 C1288

Gene Ontology

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:8eey, PDBe:8eey, PDBj:8eey
• PDBsum: 8eey
• PubMed: 36423276
• UniProt: A0A401FT36