Structure of PDB 8eex Chain A

Receptor sequence

>8eexA (length=1284) Species: 45657 (Desulfonema ishimotonii)

MTTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNT
KGRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQ
LRQRSTLRWTDKNPCPDNAETYCPFCELLGRSGNDWRFRIHFGNLSLPGK
PDFDGPKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDN
KVSAEARKLLCDSLKFTDRLCGALCVIRFNLAEKTAEQIISILDDNKKTE
YTRLLADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGKVTIRQILTTSA
DTKELKNAGKWREFCEKLGEALYLKSKDMSGGLKITRRILGDAEFHGKPD
RLEKSRSVSIGSVLKETVVCGELVAKTPFFFGAIDEDAKQTALQVLLTPD
NKYRLPRSAVRGILRRDLQTYFDSPCNAELGGRPCMCKTCRIMRGITVMD
ARSEYNAPPEIRHRTRINPFTGTVAEGALFNMEVAPEGIVFPFQLRYRGS
EDGLPDALKTVLKWWAEGQAFMSGAASTGKGRFRMENAKYETLDLSDENQ
RNDYLKNWGWRDEKGLEELKKRLNSGLPEPGNYRDPKWHEINVSIEMASP
FINGDPIRAAVDKRGTAVVTFVKYKAEGEEAKPVCAYKAESFRGVIRSAV
ARIHMEDGVPLTELTHSDCECLLCQIFGSEYEAGKIRFEDLVFESDPEPV
TFDHVAIDRFTGGAADKKKFDDSPLPGSPARPLMLKGSFWIRRDVLEDEE
YCKALGKALADVNNGLYPLGGKSAIGYGQVKSLGIKGDDKRISRLMNPAF
DETDVAVPEKPKTDAEVRIEAEKVYYPHYFVEPHKKVEREEKPCGHQKFH
EGRLTGKIRCKLITKTPLIVPDTSNDDFFRPADKEARKEKDEYHKSYAFF
RLHKQIMIPGSELRGMVSSVYETVTNSCFRIFDETKRLSWRMDADIPEKA
RIKYKELLRVYNNNPQAVDIVPVRISRTVDDRMIGKRMSADLRPCHGDWV
EDGDLSALNAYPEKRLLLRHPKGLCPACRLFGTGSYKGRVRFGFASLEND
PEWLIPGKNPGDPFHGGPVMLSLLERPRPTWSIPGSDNKFKVPGRKFYVH
HHAWKTIKDGNHPTTGKAIEQSPNNRTVEALAGGNSFSFEIAFENLKEWE
LGLLIHSLQLEKGLAHKLGMAKSMGFGSVEIDVESVRLRKDWKQWRNGNS
EIPNWLGKGFAKLKEWFRDELDFIENLKKLLWFPEGDQAPRVCYPMLRKK
DDPNGNSGYEELKDGEFKKEDRQKKLTTPWTPWA

3D structure

• PDB: 8eex RNA-activated protein cleavage with a CRISPR-associated endopeptidase.
• Chain: A
• Resolution: 2.95 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E13 R35 Q37 A38 H43 R53 Y55 T59 L60 R62 S63 R67 K89 F90 D91 T92 Q100 R102 Q103 R104 L129 W144 F146 H149 F150 N152 S154 R171 V172 L173 N174 R175 V176 G181 F186 F187 R227 F382 G384 G419 R444 S445 R448 G449 I450 R452 L467 G468 P471 R481 T484 V485 R501 T502 I504 L516 F517 G561 A562 S564 G641 P643 K675 S678 R680 V682 S716 E717 E719 D740 H741 V742 A743 I744 R746 G750 F757 G808 Y863 P908 T910 S948 E949 R951 R967 R978 Y1245 N1248 Q1250 V1290 R1291 I1292 R1294 G1349 T1350 G1351 K1354 G1355 L1391 E1392 R1393 R1395 T1397 W1398 Y1415 G1486 M1487 A1488 K1489 Y1561 L1564 Y1576 K1580	E13 R35 Q37 A38 H43 R53 Y55 T59 L60 R62 S63 R67 K89 F90 D91 T92 Q100 R102 Q103 R104 L129 W136 F138 H141 F142 N144 S146 R163 V164 L165 N166 R167 V168 G173 F178 F179 R219 F345 G347 G382 R407 S408 R411 G412 I413 R415 L430 G431 P434 R444 T447 V448 R464 T465 I467 L479 F480 G524 A525 S527 G604 P606 K638 S641 R643 V645 S679 E680 E682 D703 H704 V705 A706 I707 R709 G713 F720 G771 Y826 P871 T873 S911 E912 R914 R930 R941 Y961 N964 Q966 V973 R974 I975 R977 G1032 T1033 G1034 K1037 G1038 L1074 E1075 R1076 R1078 T1080 W1081 Y1098 G1169 M1170 A1171 K1172 Y1244 L1247 Y1259 K1263
BS02	ZN	A	C123 C126	C123 C126
BS03	ZN	A	H703 C706 C708	H666 C669 C671
BS04	ZN	A	C965 C1345	C928 C1028

Gene Ontology

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:8eex, PDBe:8eex, PDBj:8eex
• PDBsum: 8eex
• PubMed: 36423276
• UniProt: A0A401FT36