Structure of PDB 8e8b Chain A

Receptor sequence

>8e8bA (length=425) Species: 9606 (Homo sapiens)

GPHMATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGI
IAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASV
EVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYI
EGLPQGQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIE
RETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKI
RSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGI
EHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKD
RNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKN
CNTSGTEWSPPLTMLFLCATKFSAS

3D structure

• PDB: 8e8b Primer terminal ribonucleotide alters the active site dynamics of DNA polymerase eta and reduces DNA synthesis fidelity.
• Chain: A
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 W42 S62 K86 R93 K311 K317 T318 G320 S322 K323 N324 P326 R351	Q41 Y42 W45 S65 K89 R96 K306 K312 T313 G315 S317 K318 N319 P321 R346
BS02	dna	A	S257 G259 G260 K261 L262 R377 R382 R383 C384	S252 G254 G255 K256 L257 R372 R377 R378 C379
BS03	DGT	A	D15 C16 F17 F18 Q38 I48 Y52 R55 R61 D115 K231	D18 C19 F20 F21 Q41 I51 Y55 R58 R64 D118 K226
BS04	CA	A	D13 M14 D115	D16 M17 D118

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:8e8b, PDBe:8e8b, PDBj:8e8b
• PDBsum: 8e8b
• PubMed: 36702254
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)