Structure of PDB 8e2q Chain A

Receptor sequence
>8e2qA (length=154) Species: 562 (Escherichia coli) [Search protein sequence]
VEFSHEYWMRHALALAKRARDEREVPVGAVLVLNNRVIGEGWNRGIGLHD
PTAHAEIMALRQGGLVMQNYRLYDATLYTTFEPCVMCAGAMIHSRIGRVV
FGVRNAKTGAAGSLMDVLHHPGMNHRVEITEGILADECEALLCRFFRMPR
RVFN
3D structure
PDB8e2q Improved cytosine base editors generated from TadA variants.
ChainA
Resolution2.34 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.5.4.33: tRNA(adenine(34)) deaminase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A V30 N46 H57 E59 C87 N108 K110 F148 F149 R150 M151 P152 V155 F156 N157 V27 N43 H54 E56 C84 N105 K107 F145 F146 R147 M148 P149 V152 F153 N154
BS02 dna A R74 Y76 R98 R71 Y73 R95
BS03 ZN A H57 C87 C90 H54 C84 C87
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0008251 tRNA-specific adenosine deaminase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
GO:0052717 tRNA-specific adenosine-34 deaminase activity
Biological Process
GO:0002100 tRNA wobble adenosine to inosine editing
GO:0006382 adenosine to inosine editing
GO:0008033 tRNA processing

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Molecular Function
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Biological Process
External links
PDB RCSB:8e2q, PDBe:8e2q, PDBj:8e2q
PDBsum8e2q
PubMed36624149
UniProtP68398|TADA_ECOLI tRNA-specific adenosine deaminase (Gene Name=tadA)

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