Structure of PDB 8e2p Chain A

Receptor sequence
>8e2pA (length=151) Species: 562 (Escherichia coli) [Search protein sequence]
FSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPT
AHAEIMALRQGGLVMQNYRLYDATLYSTFEPCVMCAGAMIHSRIGRVVFG
VRNAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLCRFFRMPRRV
F
3D structure
PDB8e2p Improved cytosine base editors generated from TadA variants.
ChainA
Resolution2.72 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 3.5.4.33: tRNA(adenine(34)) deaminase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A V30 N46 H57 E59 F84 N108 K110 T111 F148 F149 R150 M151 P152 F156 V25 N41 H52 E54 F79 N103 K105 T106 F143 F144 R145 M146 P147 F151
BS02 dna A Y73 R74 Y76 R98 Y68 R69 Y71 R93
BS03 ZN A H57 C87 C90 H52 C82 C85
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0008251 tRNA-specific adenosine deaminase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
GO:0052717 tRNA-specific adenosine-34 deaminase activity
Biological Process
GO:0002100 tRNA wobble adenosine to inosine editing
GO:0006382 adenosine to inosine editing
GO:0008033 tRNA processing

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8e2p, PDBe:8e2p, PDBj:8e2p
PDBsum8e2p
PubMed36624149
UniProtP68398|TADA_ECOLI tRNA-specific adenosine deaminase (Gene Name=tadA)

[Back to BioLiP]