Structure of PDB 8e1i Chain A

Receptor sequence

>8e1iA (length=331) Species: 584653 (Schizosaccharomyces pombe/Puccinia graminis mixed DNA library)

RNVVGICAMDAKARSAPCANILNRIIAEGEFEAIVFGDNMILDEAVENWP
ACDYLICFYSSGFPLKKAEKYVELRKPFCVNDVVFQELLWDRRLVLNILD
AIRVSTPQRLICSRDGGPKINKVLEEKLRRKFGIEITEVPTPEVKMLDED
TLSVDGKIIKKPYVEKPVYGEDHNIYIYFPKSVGGGGRKLFRKVANKSSD
YDPDLCAPRTEGSFIYEEFMNVDNAEDVKVYTVGPHYSHAETRKSPVVDG
IVRRNPHGKEIRFITNLSEEEKNMASKISIAFEQPVCGFDLLRVSGQSYV
IDVNGWSFVKDNNDYYDNAARILKQMFHVAE

3D structure

• PDB: 8e1i Structures of Fission Yeast Inositol Pyrophosphate Kinase Asp1 in Ligand-Free, Substrate-Bound, and Product-Bound States.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.7.4.24: diphosphoinositol-pentakisphosphate 1-kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	I7P	A	K43 R223 K224 K260 R274 R285 K290 R293 K341	K12 R192 K193 K229 R243 R254 K259 R262 K310
BS02	ATP	A	R123 V195 K197 H204 I206 L221 E248 F250 M251 D258 D321 L323 I332 D333	R92 V164 K166 H173 I175 L190 E217 F219 M220 D227 D290 L292 I301 D302
BS03	MG	A	D321 D333	D290 D302
BS04	MG	A	D333 N335	D302 N304

Gene Ontology

Molecular Function

• GO:0000829 diphosphoinositol pentakisphosphate kinase activity

External links

• PDB: RCSB:8e1i, PDBe:8e1i, PDBj:8e1i
• PDBsum: 8e1i
• PubMed: 36468882
• UniProt: O74429|VIP1_SCHPO Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase (Gene Name=asp1)