Structure of PDB 8dqz Chain A

Receptor sequence

>8dqzA (length=492) Species: 4932 (Saccharomyces cerevisiae)

VVREEDKLWTVKYAPTNLQQVCGNKGSVMKLKNWLANWENSKKNSFKHAG
KDGSGVFRAAMLYGPPGIGKTTAAHLVAQELGYDILEQNASDVRSKTLLN
AGVKNALDNMSVVGYFKHNLNGKHFVIIMDEVDGMSGGDRGGVGQLAQFC
RKTSTPLILICNERNLPKMRPFDRVCLDIQFRRPDANSIKSRLMTIAIRE
KFKLDPNVIDRLIQTTRGDIRQVINLLSTISTTTKTINHENINEISKAWE
KNIALKPFDIAHKMLDGQIYSDIGSRNFTLNDKIALYFDDFDFTPLMIQE
NYLSTRPSVLKPGQSHLEAVAEAANCISLGDIVEKKIRSSEQLWSLLPLH
AVLSSVYPASKVAGHMAGRINFTAWLGQNSKSAKYYRLLQEIHYHTRLGT
STDKIGLRLDYLPTFRKRLLDPFLKQGADAISSVIEVMDDYYLTKEDWDS
IMEFFVGPDVTTAIIKKIPATVKSGFTRKYNSMTHPVAIYRT

3D structure

• PDB: 8dqz Multistep loading of a DNA sliding clamp onto DNA by replication factor C.
• Chain: A
• Resolution: 2.92 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R434 P461 F552 D586 F587 L590 R632 Q636 F666 W669 L670	R140 P167 F258 D292 F293 L296 R338 Q342 F372 W375 L376
BS02	dna	A	D433 F582 W638	D139 F288 W344
BS03	AGS	A	T299 P304 C311 P355 G356 I357 G358 K359 T360 T361 N456 I514 R515	T10 P15 C22 P66 G67 I68 G69 K70 T71 T72 N162 I220 R221

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003689 DNA clamp loader activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0000278 mitotic cell cycle
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006271 DNA strand elongation involved in DNA replication
• GO:0006272 leading strand elongation
• GO:0006281 DNA repair
• GO:0006298 mismatch repair
• GO:0051301 cell division

Cellular Component

• GO:0005634 nucleus
• GO:0005663 DNA replication factor C complex

External links

• PDB: RCSB:8dqz, PDBe:8dqz, PDBj:8dqz
• PDBsum: 8dqz
• PubMed: 35939393
• UniProt: P38630|RFC1_YEAST Replication factor C subunit 1 (Gene Name=RFC1)