Structure of PDB 8dq8 Chain A

Receptor sequence

>8dq8A (length=432) Species: 1042876 (Pseudomonas putida S16)

GFDYDVVVVGGGFAGATAARECGLQGYRTLLLEARSRLGGRTFTSRFAGQ
EIELGGTWVHWLQPHVWAEMQRYGLGVVEDPLTNLDKTLIMYNDGIVESI
SPDEFGKNIRIAFEKLCHDAWEVFPRPHEPMFTERARELDKSSVLDRIKT
LGLSRLQQAQINSYMALYAGETTDKFGLPGVLKLFACGGWNYDAFMDTET
HYRIQGGTIGLINAMLTDSGAEVRMSVPVTAVEQVNGGVKIKTDDDEIIT
AGVVVMTVPLNTYKHIDFTPALSKGKQRFIKEGQLSKGAKLYVHVKQNLG
RVFAFADEQQPLNWVQTRDYSDELGTILSITIARKETIDVNDRDAVTREV
QKMFPGVEVLGTAAYDWTADPFSLGAWAAYGVGQLSRLKDLQAAEGRIVF
AGAETSNGWHASIDGAVESGLRAGREVKQLLS

3D structure

• PDB: 8dq8 Directed evolution unlocks oxygen reactivity for a nicotine degrading flavoenzyme
• Chain: A
• Resolution: 1.9 Å

Enzymatic activity

• Enzyme Commision number: 1.4.2.2: nicotine dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NCT	A	Y218 E249 T250 W364 T381 W427 A461	Y168 E199 T200 W314 T331 W377 A411
BS02	FDA	A	V59 G60 G62 A64 E83 A84 R85 G90 R91 G105 G106 T107 W108 V279 V308 P309 W417 F422 A426 W427 G452 A453 S462 I463 A466	V9 G10 G12 A14 E33 A34 R35 G40 R41 G55 G56 T57 W58 V229 V258 P259 W367 F372 A376 W377 G402 A403 S412 I413 A416

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0009820 alkaloid metabolic process
• GO:0019608 nicotine catabolic process

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:8dq8, PDBe:8dq8, PDBj:8dq8
• PDBsum: 8dq8
• PubMed: 37770699
• UniProt: F8G0P2|NICA2_PSEP6 Nicotine dehydrogenase (Gene Name=nicA2)