Structure of PDB 8dp2 Chain A

Receptor sequence

>8dp2A (length=446) Species: 208964 (Pseudomonas aeruginosa PAO1)

HHHHHMFRIVVGLGKSGMSLVRYLARRGLPFAVVDTRENPPELATLRAQY
PQVEVRCGELDAEFLCSARELYVSPGLSLRTPALVQAAAKGVRISGDIDL
FAREAKAPIVAITGSNAKSTVTTLVGEMAVAADKRVAVGGNLGTPALDLL
ADDIELYVLELSSFQLETCDRLNAEVATVLNVSEDHMDRYDGMADYHLAK
HRIFRGARQVVVNRADALTRPLIADTVPCWSFGLNKPDFKAFGLIEEDGQ
KWLAFQFDKLLPVGELKIRGAHNYSNALAALALGHAVGLPFDAMLGALKA
FSGLAHRCQWVRERQGVSYYDDSKATNVGAALAAIEGLGADIDGKLVLLA
GGDGKGADFHDLREPVARFCRAVVLLGRDAGLIAQALGNAVPLVRVATLD
EAVRQAAELAREGDAVLLSPACASLDMFKNFEERGRLFAKAVEELA

3D structure

• PDB: 8dp2 Crystal Structure of UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from Pseudomonas aeruginosa PAO1 in complex with UMA (Uridine-5'-diphosphate-N-acetylmuramoyl-L-Alanine)
• Chain: A
• Resolution: 1.6 Å

Enzymatic activity

• Enzyme Commision number: 6.3.2.9: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UMA	A	K17 S18 D37 T38 R39 S76 P77 G78 G142 N143 F166 H188	K15 S16 D35 T36 R37 S74 P75 G76 G140 N141 F164 H186

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008764 UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
• GO:0016874 ligase activity
• GO:0016881 acid-amino acid ligase activity

Biological Process

• GO:0008360 regulation of cell shape
• GO:0009058 biosynthetic process
• GO:0009252 peptidoglycan biosynthetic process
• GO:0051301 cell division
• GO:0071555 cell wall organization

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8dp2, PDBe:8dp2, PDBj:8dp2
• PDBsum: 8dp2
• UniProt: Q9HVZ9|MURD_PSEAE UDP-N-acetylmuramoylalanine--D-glutamate ligase (Gene Name=murD)