Structure of PDB 8d2q Chain A

Receptor sequence

>8d2qA (length=906) Species: 351607 (Acidothermus cellulolyticus 11B) [Search protein sequence]

GTVPVTWRLGVDVGERSIGLAAVSYEEDKPKEILAAVSWIHDGGVGDERS
GASRLALRGMARRARRLRRFRRARLRDLDMLLSELGWTPLPDKNVSPVDA
WLARKRLAEEYVVDETERRRLLGYAVSHMARHRGWRNPWTTIKDLKNLPQ
PSDSWERTRESLEARYSVSLEPGTVGQWAGYLLQRAPGIRLNPTQQSLSN
ATAFETRLRQEDVLWELRCIADVQGLPEDVVSNVIDAVFCQKRPSVPAER
IRPPLPALHEATGHPVVDRNLAILRKFLSSATMRWGPPQSIVVELARGAS
ESRERQAEEEAARRAHRKANDRIRAELRASGLSDPSPADLVRARLLELYD
CHCMYCGAPISWENSELDHIVPRTDGGSNRHENLAITCGACNKEKGRRPF
ASWAETSNRVQLRDVIDRVQKLKYSGNMYWTRDEFSRYKKSVVARLKRRT
SDPEVIQSIESTGYAAVALRDRLLSYGEKNGVAQVAVFRGGVTAEARRWL
DISIERLFSRVAIFAQSTSTKRLDRRHHAVDAVVLTTLTPGVAKTLADAR
SRRVSAEFWRRPSTEEPQSPAYRQWKESCSGLGDLLISTAARDSIAVAAP
LRLRPTGALHEETLRAFSEHTVGAAWKGAELRRIVEPEVYAAFLALTDPG
GRFLKVSPSEDVLPADENRHIVLSDRVLGPRDRVKLFPDDRGSIRVRGGA
AYIASFHHARVFRWGSSHSPSFALLRVSLADLAVAGLLRDGVDVFTAELP
PWTPAWRYASIALVKAVESGDAKQVGWLVPGDELDFGPEGVTTAAGDLSM
FLKYFPERHWVVTGFEDDKRINLKPAFLSAEQAEVLRTERSDRPDTLTEA
GEILAQFFPRCWRATVAKVLCHPGLTVIRRTALGQPRWRRGHLPYSWRPW
SADPWS

3D structure

PDB

8d2q Coupled catalytic states and the role of metal coordination in Cas9

Chain

Resolution

2.58 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	H47 D48 S59 R60 L61 A62 R64 G65 R68 R69 R71 R72 L73 R74 R75 F76 R78 R82 L96 D98 K99 V101 S102 P103 W107 H134 R137 H138 R139 G140 W141 P144 P193 G194 I195 R196 L197 N198 Q201 Q253 K254 P256 S257 P259 E261 H481 R491 R497 K498 S501 S502 R506 S524 P828 L829 R830 L831 R832 T834 G835 A836 L837 H838 L842 R843 A844 R925 G969 V970 D971 V972 F973 T1109 L1111 Q1113 P1114 R1115 W1116 R1117 H1120 P1122	H41 D42 S53 R54 L55 A56 R58 G59 R62 R63 R65 R66 L67 R68 R69 F70 R72 R76 L90 D92 K93 V95 S96 P97 W101 H128 R131 H132 R133 G134 W135 P138 P187 G188 I189 R190 L191 N192 Q195 Q241 K242 P244 S245 P247 E249 H259 R269 R275 K276 S279 S280 R284 S302 P600 L601 R602 L603 R604 T606 G607 A608 L609 H610 L614 R615 A616 R697 G741 V742 D743 V744 F745 T881 L883 Q885 P886 R887 W888 R889 H892 P894
BS02	dna	A	W141 N143 W145 R219 I263 T684 Y686 V689	W135 N137 W139 R207 I251 T462 Y464 V467
BS03	dna	A	R55 Q201 Q202 E839 E840 T841 D1025 R1048 R1088 R1091	R49 Q195 Q196 E611 E612 T613 D797 R820 R860 R863
BS04	dna	A	E54 R55 D918 R919 F934 R954 G1042 F1043 E1044	E48 R49 D690 R691 F706 R726 G814 F815 E816
BS05	MG	A	D18 H749 H750	D12 H527 H528

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0008270	zinc ion binding
GO:0046872	metal ion binding

	Biological Process
GO:0051607	defense response to virus

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:8d2q, PDBe:8d2q, PDBj:8d2q
PDBsum	8d2q
PubMed	38348449
UniProt	A0LWB3

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