Structure of PDB 8czq Chain A

Receptor sequence

>8czqA (length=655) Species: 1773 (Mycobacterium tuberculosis) [Search protein sequence]

SGRRLVIVESPTKARKLASYLGSGYIVESSRGHIRDLPRAASDVPAKYKS
QPWARLGVNVDADFEPLYIISPEKRSTVSELRGLLKDVDELYLATDGDRE
GEAIAWHLLETLKPRIPVKRMVFHEITEPAIRAAAEHPRDLDIDLVDAQE
TRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVARERDRMAFRS
AAYWDILAKLTFSARLTAVAGRRVATGRDFDSLGTLRKEVIVLDEGSATA
LAADGTQLTVASAEEKPYARRPYPPFMTSTLQQEASRKLRFSAERTMSIA
QRLYENGYITYMRTDSTTLSESAINAARTQARQLYGDEYVAPAPRQYTRK
VKQEAHEAIRPAGETFATPDAVRRELDGPNIDDFRLYELIWQRTVASQMA
DARGMTLSLRITGMSGHQEVVFSATGRTLTFPGFLKAYVRLPHLTPGQRL
DIVELTPDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTIQDRGY
VHKKGSALVPSWVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIAAGNER
RTNWLNNFYFGGDHGVPDSVARSGGLKKLVGINLEGIDAREVNSIKLFDD
THGRPIYVRVGKNGPYLERLVAGDTGEPTPQRANLSDSITPDELTLQVAE
ELFAT

3D structure

PDB

8czq The interaction between transport-segment DNA and topoisomerase IA-crystal structure of MtbTOP1 in complex with both G- and T-segments.

Chain

Resolution

2.78 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	S25 P26 T27 A29 R30 E43 S44 R46 G47 H48 D51 R54 K89 E95 R167 R168 D171 R172 G175 Y176 S179 W183 P188 K189 S191 A192 G193 R194 V195 Q196 Q332 Y342 R344 R533 S535 T536	S10 P11 T12 A14 R15 E28 S29 R31 G32 H33 D36 R39 K74 E80 R152 R153 D156 R157 G160 Y161 S164 W168 P173 K174 S176 A177 G178 R179 V180 Q181 Q301 Y311 R313 R484 S486 T487
BS02	dna	A	Y299 R301 Y304 R318 R460 T518 K553 G554 S555	Y268 R270 Y273 R287 R427 T469 K504 G505 S506

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003916	DNA topoisomerase activity
GO:0003917	DNA topoisomerase type I (single strand cut, ATP-independent) activity

	Biological Process
GO:0006265	DNA topological change

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:8czq, PDBe:8czq, PDBj:8czq
PDBsum	8czq
PubMed	36583363
UniProt	P9WG49\|TOP1_MYCTU DNA topoisomerase 1 (Gene Name=topA)

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