Structure of PDB 8cm2 Chain A

Receptor sequence

>8cm2A (length=460) Species: 4577 (Zea mays)

GGRLSVDASDIAEASRDFGGVARAEPMAVFHPRAAGDVAGLVGAAFRSAR
GFRVSARGHGHSISGQAQAAGGVVVDMSRGVARALPVHSAALGGHYVDVW
GGELWVDVLNWTLSHGGLAPRSWTDYLYLSVGGTLSNAGISGQAFHHGPQ
ISNVYELDVVTGKGEVVTCSETENPDLFFGVLGGLGQFGIITRARIALER
APKRVRWIRALYSNFSEFTADQERLISLGSGGGRRFDYVEGFVVAAEHSS
VLYCLEVTKNYDDETAGSVDQDVDTLLGELNFLPGTVFTTDLPYVDFLDR
VHKAELKLRAKGMWEVPHPWLNLFVPASRIADFDRGVFRGVLGGRTAGGP
VLIYPMNKHKWDPRSSAVTPDEEVFYLVAFLRSALPGAPESLEALARQNQ
RILDFCAGTGIGAKQYLPGHKARHEWAEHFGAARWDRFARLKAEFDPRAI
LAAGQGIFRP

3D structure

• PDB: 8cm2 Cytokinin oxidase/dehydrogenase inhibitors: progress toward a practice
• Chain: A
• Resolution: 2.05 Å

Enzymatic activity

• Enzyme Commision number: 1.5.99.12: cytokinin dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	V1X	A	D170 V370 L377 W389 N391 L423 Y425 L448 L452 Y487	D125 V301 L308 W320 N322 L352 Y354 L377 L381 Y416
BS02	FAD	A	F57 A95 R96 G97 H98 G99 H100 S101 Q105 A106 T169 D170 Y171 L174 S175 G177 G178 S181 N182 I185 I235 I236 W389 Y487	F18 A56 R57 G58 H59 G60 H61 S62 Q66 A67 T124 D125 Y126 L129 S130 G132 G133 S136 N137 I140 I190 I191 W320 Y416

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0019139 cytokinin dehydrogenase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0009690 cytokinin metabolic process

External links

• PDB: RCSB:8cm2, PDBe:8cm2, PDBj:8cm2
• PDBsum: 8cm2
• PubMed: 38776394
• UniProt: E3T1W8