Structure of PDB 8cfp Chain A

Receptor sequence

>8cfpA (length=464) Species: 208964 (Pseudomonas aeruginosa PAO1)

TPAGFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAK
ILGCIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPV
FAWKGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQML
ERIHGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRH
SLNDAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPI
CAMQACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCD
ANMLKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKD
GFDAHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQK
YADLPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIG
VSVEGPFKPDTYRY

3D structure

• PDB: 8cfp Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry F09
• Chain: A
• Resolution: 1.61 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	T165 T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 G299 N300 I321 G322 H323 N375 H382	T160 T161 T162 N194 I222 G225 D226 V227 E246 V247 T293 G294 N295 I316 G317 H318 N370 H377
BS02	ADE	A	H61 T63 Q65 H382 M387	H56 T58 Q60 H377 M382
BS03	UI4	A	L396 H400 L423 P424 L427	L391 H395 L418 P419 L422
BS04	UI4	A	K200 K234 Q238 D263	K195 K229 Q233 D258
BS05	NAD	A	K463 Y467	K458 Y462

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8cfp, PDBe:8cfp, PDBj:8cfp
• PDBsum: 8cfp
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)