Structure of PDB 8cfo Chain A

Receptor sequence

>8cfoA (length=462) Species: 208964 (Pseudomonas aeruginosa PAO1)

AGFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKIL
GCIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFA
WKGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLER
IHGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSL
NDAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICA
MQACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDAN
MLKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGF
DAHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYA
DLPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVS
VEGPFKPDTYRY

3D structure

• PDB: 8cfo Crystal structure of S-adenosyl-L-homocysteine hydrolase from P. aeruginosa in complex with fragment F2X-Entry F04
• Chain: A
• Resolution: 2.13 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	A	T165 T166 T167 N199 G230 D231 V232 E251 V252 T298 N300 I321 G322 H323 N375 H382	T158 T159 T160 N192 G223 D224 V225 E244 V245 T291 N293 I314 G315 H316 N368 H375
BS02	ADE	A	H61 T63 L376 H382 M387	H54 T56 L369 H375 M380
BS03	RB7	A	G40 L41 K44 E430 V431	G33 L34 K37 E423 V424
BS04	RB7	A	I186 H400 P424 L427	I179 H393 P417 L420
BS05	RB7	A	Y116 E117 D142 I146 R171 F324	Y109 E110 D135 I139 R164 F317
BS06	RB7	A	E113 E114 E117 I146	E106 E107 E110 I139
BS07	RB7	A	K14 W108 E115 W118	K7 W101 E108 W111
BS08	NAD	A	K463 Y467	K456 Y460

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8cfo, PDBe:8cfo, PDBj:8cfo
• PDBsum: 8cfo
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)